LOCUS       Exported                7400 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 
            (aa 2-2414) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-dCas9-FLp300
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3206..5056
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      4820..4829
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7307..7333
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7358..7375)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gctaggaggt gacccaattg ccggatccaa ggctagcccg aaaaagaaac gcaaagttgg
       61 gcgcgccgcc gagaatgtgg tggaaccggg gccgccttca gccaagcggc ctaaactctc
      121 atctccggcc ctctcggcgt ccgccagcga tggcacagat tttggctctc tatttgactt
      181 ggagcacgac ttaccagatg aattaatcaa ctctacagaa ttgggactaa ccaatggtgg
      241 tgatattaat cagcttcaga caagtcttgg catggtacaa gatgcagctt ctaaacataa
      301 acagctgtca gaattgctgc gatctggtag ttcccctaac ctcaatatgg gagttggtgg
      361 cccaggtcaa gtcatggcca gccaggccca acagagcagt cctggattag gtttgataaa
      421 tagcatggtc aaaagcccaa tgacacaggc aggcttgact tctcccaaca tggggatggg
      481 cactagtgga ccaaatcagg gtcctacgca gtcaacaggt atgatgaaca gtccagtaaa
      541 tcagcctgcc atgggaatga acacagggat gaatgcgggc atgaatcctg gaatgttggc
      601 tgcaggcaat ggacaaggga taatgcctaa tcaagtcatg aacggttcaa ttggagcagg
      661 ccgagggcga cagaatatgc agtacccaaa cccaggcatg ggaagtgctg gcaacttact
      721 gactgagcct cttcagcagg gctctcccca gatgggagga caaacaggat tgagaggccc
      781 ccagcctctt aagatgggaa tgatgaacaa ccccaatcct tatggttcac catatactca
      841 gaatcctgga cagcagattg gagccagtgg ccttggtctc cagattcaga caaaaactgt
      901 actatcaaat aacttatctc catttgctat ggacaaaaag gcagttcctg gtggaggaat
      961 gcccaacatg ggtcaacagc cagccccgca ggtccagcag ccaggtctgg tgactccagt
     1021 tgcccaaggg atgggttctg gagcacatac agctgatcca gagaagcgca agctcatcca
     1081 gcagcagctt gttctccttt tgcatgctca caagtgccag cgccgggaac aggccaatgg
     1141 ggaagtgagg cagtgcaacc ttccccactg tcgcacaatg aagaatgtcc taaaccacat
     1201 gacacactgc cagtcaggca agtcttgcca agtggcacac tgtgcatctt ctcgacaaat
     1261 catttcacac tggaagaatt gtacaagaca tgattgtcct gtgtgtctcc ccctcaaaaa
     1321 tgctggtgat aagagaaatc aacaaccaat tttgactgga gcacccgttg gacttggaaa
     1381 tcctagctct ctaggggtgg gtcaacagtc tgcccccaac ctaagcactg ttagtcagat
     1441 tgatcccagc tccatagaaa gagcctatgc agctcttgga ctaccctatc aagtaaatca
     1501 gatgccgaca caaccccagg tgcaagcaaa gaaccagcag aatcagcagc ctgggcagtc
     1561 tccccaaggc atgcggccca tgagcaacat gagtgctagt cctatgggag taaatggagg
     1621 tgtaggagtt caaacgccga gtcttctttc tgactcaatg ttgcattcag ccataaattc
     1681 tcaaaaccca atgatgagtg aaaatgccag tgtgccctcc atgggtccta tgccaacagc
     1741 agctcaacca tccactactg gaattcggaa acagtggcac gaagatatta ctcaggatct
     1801 tcgaaatcat cttgttcaca aactcgtcca agccatattt cctacgccgg atcctgctgc
     1861 tttaaaagac agacggatgg aaaacctagt tgcatatgct cggaaagttg aaggggacat
     1921 gtatgaatct gcaaacaatc gagcggaata ctaccacctt ctagctgaga aaatctataa
     1981 gatccagaaa gaactagaag aaaaacgaag gaccagacta cagaagcaga acatgctacc
     2041 aaatgctgca ggcatggttc cagtttccat gaatccaggg cctaacatgg gacagccgca
     2101 accaggaatg acttctaatg gccctctacc tgacccaagt atgatccgtg gcagtgtgcc
     2161 aaaccagatg atgcctcgaa taactccaca atctggtttg aatcaatttg gccagatgag
     2221 catggcccag ccccctattg taccccggca aacccctcct cttcagcacc atggacagtt
     2281 ggctcaacct ggagctctca acccgcctat gggctatggg cctcgtatgc aacagccttc
     2341 caaccagggc cagttccttc ctcagactca gttcccatca cagggaatga atgtaacaaa
     2401 tatccctttg gctccgtcca gcggtcaagc tccagtgtct caagcacaaa tgtctagttc
     2461 ttcctgcccg gtgaactctc ctataatgcc tccagggtct caggggagcc acattcactg
     2521 tccccagctt cctcaaccag ctcttcatca gaattcaccc tcgcctgtac ctagtcgtac
     2581 ccccacccct caccatactc ccccaagcat aggggctcag cagccaccag caacaacaat
     2641 tccagcccct gttcctacac ctcctgccat gccacctggg ccacagtccc aggctctaca
     2701 tccccctcca aggcagacac ctacaccacc aacaacacaa cttccccaac aagtgcagcc
     2761 ttcacttcct gctgcacctt ctgctgacca gccccagcag cagcctcgct cacagcagag
     2821 cacagcagcg tctgttccta ccccaacagc accgctgctt cctccgcagc ctgcaactcc
     2881 actttcccag ccagctgtaa gcattgaagg acaggtatca aatcctccat ctactagtag
     2941 cacagaagtg aattctcagg ccattgcgga gaagcagcct tcccaggaag tgaagatgga
     3001 ggccaaaatg gaagtggatc aaccagaacc agcagatact cagccggagg atatttcaga
     3061 gtctaaagtg gaagactgta aaatggaatc taccgaaaca gaagagagaa gcactgagtt
     3121 aaaaactgaa ataaaagagg aggaagacca gccaagtact tcagctaccc agtcatctcc
     3181 ggctccagga cagtcaaaga aaaagatttt caaaccagaa gaactacgac aggcactgat
     3241 gccaactttg gaggcacttt accgtcagga tccagaatcc cttccctttc gtcaacctgt
     3301 ggaccctcag cttttaggaa tccctgatta ctttgatatt gtgaagagcc ccatggatct
     3361 ttctaccatt aagaggaagt tagacactgg acagtatcag gagccctggc agtatgtcga
     3421 tgatatttgg cttatgttca ataatgcctg gttatataac cggaaaacat cacgggtata
     3481 caaatactgc tccaagctct ctgaggtctt tgaacaagaa attgacccag tgatgcaaag
     3541 ccttggatac tgttgtggca gaaagttgga gttctctcca cagacactgt gttgctacgg
     3601 caaacagttg tgcacaatac ctcgtgatgc cacttattac agttaccaga acaggtatca
     3661 tttctgtgag aagtgtttca atgagatcca aggggagagc gtttctttgg gggatgaccc
     3721 ttcccagcct caaactacaa taaataaaga acaattttcc aagagaaaaa atgacacact
     3781 ggatcctgaa ctgtttgttg aatgtacaga gtgcggaaga aagatgcatc agatctgtgt
     3841 ccttcaccat gagatcatct ggcctgctgg attcgtctgt gatggctgtt taaagaaaag
     3901 tgcacgaact aggaaagaaa ataagttttc tgctaaaagg ttgccatcta ccagacttgg
     3961 cacctttcta gagaatcgtg tgaatgactt tctgaggcga cagaatcacc ctgagtcagg
     4021 agaggtcact gttagagtag ttcatgcttc tgacaaaacc gtggaagtaa aaccaggcat
     4081 gaaagcaagg tttgtggaca gtggagagat ggcagaatcc tttccatacc gaaccaaagc
     4141 cctctttgcc tttgaagaaa ttgatggtgt tgacctgtgc ttctttggca tgcatgttca
     4201 agagtatggc tctgactgcc ctccacccaa ccagaggaga gtatacatat cttacctcga
     4261 tagtgttcat ttcttccgtc ctaaatgctt gaggactgca gtctatcatg aaatcctaat
     4321 tggatattta gaatatgtca agaaattagg ttacacaaca gggcatattt gggcatgtcc
     4381 accaagtgag ggagatgatt atatcttcca ttgccatcct cctgaccaga agatacccaa
     4441 gcccaagcga ctgcaggaat ggtacaaaaa aatgcttgac aaggctgtat cagagcgtat
     4501 tgtccatgac tacaaggata tttttaaaca agctactgaa gatagattaa caagtgcaaa
     4561 ggaattgcct tatttcgagg gtgatttctg gcccaatgtt ctggaagaaa gcattaagga
     4621 actggaacag gaggaagaag agagaaaacg agaggaaaac accagcaatg aaagcacaga
     4681 tgtgaccaag ggagacagca aaaatgctaa aaagaagaat aataagaaaa ccagcaaaaa
     4741 taagagcagc ctgagtaggg gcaacaagaa gaaacccggg atgcccaatg tatctaacga
     4801 cctctcacag aaactatatg ccaccatgga gaagcataaa gaggtcttct ttgtgatccg
     4861 cctcattgct ggccctgctg ccaactccct gcctcccatt gttgatcctg atcctctcat
     4921 cccctgcgat ctgatggatg gtcgggatgc gtttctcacg ctggcaaggg acaagcacct
     4981 ggagttctct tcactccgaa gagcccagtg gtccaccatg tgcatgctgg tggagctgca
     5041 cacgcagagc caggaccgct ttgtctacac ctgcaatgaa tgcaagcacc atgtggagac
     5101 acgctggcac tgtactgtct gtgaggatta tgacttgtgt atcacctgct ataacactaa
     5161 aaaccatgac cacaaaatgg agaaactagg ccttggctta gatgatgaga gcaacaacca
     5221 gcaggctgca gccacccaga gcccaggcga ttctcgccgc ctgagtatcc agcgctgcat
     5281 ccagtctctg gtccatgctt gccagtgtcg gaatgccaat tgctcactgc catcctgcca
     5341 gaagatgaag cgggttgtgc agcataccaa gggttgcaaa cggaaaacca atggcgggtg
     5401 ccccatctgc aagcagctca ttgccctctg ctgctaccat gccaagcact gccaggagaa
     5461 caaatgcccg gtgccgttct gcctaaacat caagcagaag ctccggcagc aacagctgca
     5521 gcaccgacta cagcaggccc aaatgcttcg caggaggatg gccagcatgc agcggactgg
     5581 tgtggttggg cagcaacagg gcctcccttc ccccactcct gccactccaa cgacaccaac
     5641 tggccaacag ccaaccaccc cgcagacgcc ccagcccact tctcagcctc agcctacccc
     5701 tcccaatagc atgccaccct acttgcccag gactcaagct gctggccctg tgtcccaggg
     5761 taaggcagca ggccaggtga cccctccaac ccctcctcag actgctcagc caccccttcc
     5821 agggccccca cctgcagcag tggaaatggc aatgcagatt cagagagcag cggagacgca
     5881 gcgccagatg gcccacgtgc aaatttttca aaggccaatc caacaccaga tgcccccgat
     5941 gactcccatg gcccccatgg gtatgaaccc acctcccatg accagaggtc ccagtgggca
     6001 tttggagcca gggatgggac cgacagggat gcagcaacag ccaccctgga gccaaggagg
     6061 attgcctcag ccccagcaac tacagtctgg gatgccaagg ccagccatga tgtcagtggc
     6121 ccagcatggt caacctttga acatggctcc acaaccagga ttgggccagg taggtatcag
     6181 cccactcaaa ccaggcactg tgtctcaaca agccttacaa aaccttttgc ggactctcag
     6241 gtctcccagc tctcccctgc agcagcaaca ggtgcttagt atccttcacg ccaaccccca
     6301 gctgttggct gcattcatca agcagcgggc tgccaagtat gccaactcta atccacaacc
     6361 catccctggg cagcctggca tgccccaggg gcagccaggg ctacagccac ctaccatgcc
     6421 aggtcagcag ggggtccact ccaatccagc catgcagaac atgaatccaa tgcaggcggg
     6481 cgttcagagg gctggcctgc cccagcagca accacagcag caactccagc cacccatggg
     6541 agggatgagc ccccaggctc agcagatgaa catgaaccac aacaccatgc cttcacaatt
     6601 ccgagacatc ttgagacgac agcaaatgat gcaacagcag cagcaacagg gagcagggcc
     6661 aggaataggc cctggaatgg ccaaccataa ccagttccag caaccccaag gagttggcta
     6721 cccaccacag cagcagcagc ggatgcagca tcacatgcaa cagatgcaac aaggaaatat
     6781 gggacagata ggccagcttc cccaggcctt gggagcagag gcaggtgcca gtctacaggc
     6841 ctatcagcag cgactccttc agcaacagat ggggtcccct gttcagccca accccatgag
     6901 cccccagcag catatgctcc caaatcaggc ccagtcccca cacctacaag gccagcagat
     6961 ccctaattct ctctccaatc aagtgcgctc tccccagcct gtcccttctc cacggccaca
     7021 gtcccagccc ccccactcca gtccttcccc aaggatgcag cctcagcctt ctccacacca
     7081 cgtttcccca cagacaagtt ccccacatcc tggactggta gctgcccagg ccaaccccat
     7141 ggaacaaggg cattttgcca gcccggacca gaattcaatg ctttctcagc ttgctagcaa
     7201 tccaggcatg gcaaacctcc atggtgcaag cgccacggac ctgggactca gcaccgataa
     7261 ctcagacttg aattcaaacc tctcacagag tacactagac atacactacc cgtacgacgt
     7321 tccggactac gcttcttgag tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc
     7381 cagccatctg ttgtttgccc
//
