LOCUS       sequence-432192-       12273 bp DNA     circular SYN 27-AUG-2024
DEFINITION  Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12273)
  TITLE     Engineering Programmable Material-To-Cell Pathways Via Synthetic 
            Notch Receptors To Spatially Control Cellular Phenotypes In 
            Multi-Cellular Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2024 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..12273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    889..1113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             1213..1446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1471..1489)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1472..1489)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1779..1798)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2012..2031)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2131..2153
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2191..2209)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2277..2381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2382..3242
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2600..2619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3413..4001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3902..3921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4155..4172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4242..4262)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4247..4576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4427..4562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5710..5775
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5905..5925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6350..6484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6387..6406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6441..6460
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6652..7285
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7332..7457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7954..8187
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8372..8416
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8701..8818
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     9044..9068
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9150..9159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10170..10226
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10227..10928
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    10964..11552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11017..11037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11435..11446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11614..12113
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11649..11672)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12046..12065
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(12130..12273,1..882)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(12130..12273,1..882)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
       61 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
      121 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
      181 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
      241 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
      301 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
      361 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
      421 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
      481 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
      541 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
      601 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
      661 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
      721 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
      781 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
      841 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aggtcgagct gtgccttcta
      901 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      961 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1021 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     1081 gcaggcatgc tggggatgcg gtgggctcta tggtcgacct cgagggaatt aattcgagct
     1141 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     1201 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     1261 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     1321 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     1381 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1441 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     1501 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     1561 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     1621 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     1681 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     1741 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     1801 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     1861 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     1921 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     1981 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2041 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2101 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     2161 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     2221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2521 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2581 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2641 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2701 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2761 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2821 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2881 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2941 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3001 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3061 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3121 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3181 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3241 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4201 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     4261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4321 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4381 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4561 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     4621 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     4681 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     4741 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     4801 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     4861 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     4921 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     4981 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     5041 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     5101 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     5161 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     5221 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     5281 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     5341 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     5401 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     5461 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     5521 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     5581 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     5641 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     5701 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     5761 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     5821 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     5881 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     5941 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     6001 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     6061 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     6121 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     6181 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     6241 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6301 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6481 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     6541 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     6601 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     6661 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6721 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     6781 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     6841 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6901 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     6961 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7021 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7081 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7201 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7261 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7321 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7381 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7441 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     7501 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     7561 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     7621 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     7681 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     7741 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     7801 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7861 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7921 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7981 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8041 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8101 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8161 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8221 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8281 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8341 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8401 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8461 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8521 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8581 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     8641 atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa
     8701 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8761 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     8821 ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcgg
     8881 agcactgtcc tccgaacgtc ggagcactgt cctccgaacg tcggagcact gtcctccgaa
     8941 cgtcggagca ctgtcctccg aacggagcat gtcctccgaa cgtcggagca ctgtcctccg
     9001 aacgactagt taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     9061 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     9121 gatccagcct ctcgacattc gttggatccg ccaccatgga cctgtggaac tgggatgagg
     9181 cgtcactgca ggaagtgcct cctggggaca agctgacagg actgggagcg gaatttggtt
     9241 tctatttccc tgaagtggct ctacaagagg acacaccgat cacaccaatg aacgtagaag
     9301 gctgctggaa agggttccca gagctggact ggaaccccgc tttacctcac gaagacgtac
     9361 ctttccaggc ggagcccgtt gctcaccccc ttccgtggtc gcgagactgg acagacctgg
     9421 gatgcaacac ctcggacccg tggagctgtg cttcacagac gccaggccct gcccctcctg
     9481 gcacgagccc ctcccccttc gtcggctttg aaggggcgac cggccagaat cctgccacct
     9541 cggcaggagg ggtcccctcg tggtcgcacc ctccagctgc ctggagcact accagctggg
     9601 actgttctgt gggccccagt ggcgccacct actgggacaa tggcctgggc ggggaagcgc
     9661 atgaggacta taaaatgtca tggggcgggt ctgccggttc ggactacacc accacgtgga
     9721 atactgggct gcaggactgc agcatccctt tcgaggggca ccagagtcca gcattcacca
     9781 cgccctccaa atcgaacaag cagtctgata gagccacatt gactcgctac tccaaaacta
     9841 accaccgagg tcccattcag ctgtggcaat tcctcctgga gctgctccac gacggggctc
     9901 gcagcagctg catccgctgg acgggcaata gccgcgagtt ccagctgtgc gaccccaaag
     9961 aggtggcccg gctgtggggc gagcgcaaga ggaagccggg aatgaattat gagaaactga
    10021 gtcgaggtct acgttattat taccgccgcg acatcgtgct caagagtggt gggcgcaagt
    10081 acacataccg cttcggggga cgtgtgcctg tcctcgccta tcaggatgat atggggcatc
    10141 tgccaggtgc agaaggccaa ggaagcggag ctactaactt cagcctgctg aagcaggctg
    10201 gagacgtgga ggagaaccct ggacctatga gcgagctgat taaggagaac atgcacatga
    10261 agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag
    10321 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
    10381 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc
    10441 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga
    10501 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
    10561 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg
    10621 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg
    10681 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg
    10741 caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg
    10801 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg
    10861 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc
    10921 ttaattaatg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
    10981 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    11041 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    11101 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    11161 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    11221 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    11281 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    11341 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    11401 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    11461 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    11521 gagtcggatc tccctttggg ccgcctcccc gcgcggccgc aactcccacc tgcaacatgc
    11581 gtgactgact gaggccgcga ctctagagtc gacgggtagg ggaggcgctt ttcccaaggc
    11641 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
    11701 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
    11761 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
    11821 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
    11881 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
    11941 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
    12001 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
    12061 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacgtcga
    12121 gtcgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
    12181 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
    12241 agcttcgatg taggagggcg tggatatgtc ctg
//
