LOCUS       sequence-464645-        2643 bp DNA     circular SYN 14-MAR-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_KanR-NeoR-neo_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera 
            Xanthobacter and Roseixanthobacter
  JOURNAL   ACS Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806.
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806."
FEATURES             Location/Qualifiers
     source          1..2643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(709..728)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             720..1535
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        1621..1712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1713..2573
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2577..2616
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggct gtgtctcaaa atctctgatg ttacattgca caagataaaa
      661 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      721 tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg
      781 cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt
      841 atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg
      901 tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc
      961 tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga
     1021 ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg
     1081 tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt
     1141 ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg
     1201 tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag
     1261 aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc
     1321 tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg
     1381 gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc
     1441 cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac
     1501 tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg cggtccgcgc
     1561 gttgtcaacg tgagaccaga gtagcccgcc taatgagcgg gctttttttt aattccccta
     1621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1681 aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc
     1741 tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga
     1801 aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga
     1861 acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct
     1921 ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg
     1981 gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac
     2041 atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata
     2101 acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc
     2161 tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag
     2221 cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta
     2281 aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg
     2341 aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg
     2401 cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg
     2461 atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg
     2521 aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata
     2581 caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcaga tcaaaggatc
     2641 ttc
//
