LOCUS       sequence-464688-        1779 bp DNA     circular SYN 14-MAR-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_aadA-Broad_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera 
            Xanthobacter and Roseixanthobacter
  JOURNAL   ACS Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806.
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806."
FEATURES             Location/Qualifiers
     source          1..1779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        757..848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             849..1709
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1713..1752
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgg aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      661 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctaatgtgag accagagtag
      721 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
      781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      841 ggaagagtat gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt
      901 gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac
      961 tgggtgcgcg cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt
     1021 ttcgtccgga agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg
     1081 ttctgagccg tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga
     1141 acgatctggt ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc
     1201 gtgaactgtg cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga
     1261 cgaccattgg cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga
     1321 cccgtctgga tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata
     1381 ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga
     1441 ccctggcaag ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc
     1501 tgctgcgtag cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac
     1561 gtggcagccg tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg
     1621 tgatttatac caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa
     1681 ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc
     1741 tttttttttg gacgaccctt gtcagatcaa aggatcttc
//
