LOCUS       sequence-464819-        1770 bp DNA     circular SYN 14-MAR-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_aacC1_PartType7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera 
            Xanthobacter and Roseixanthobacter
  JOURNAL   ACS Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806.
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806."
FEATURES             Location/Qualifiers
     source          1..1770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        748..839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             840..1700
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1704..1743
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatcca ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt
      661 tcggtaaatt gtcacaacgc cgccaggtgg attgctgtga gaccagagta gcccgcctaa
      721 tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt
      781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      841 tgagcattca gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg
      901 tgtttgcgca tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc
      961 gcgtgggcta tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg
     1021 aagaacgttt tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc
     1081 gtgtggatgc gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg
     1141 tggaatatag cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt
     1201 gcagcgcggc gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg
     1261 gcggtccgaa agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg
     1321 atcgttggga accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc
     1381 cggcagcaat ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa
     1441 gccgccagca actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta
     1501 gcgcgctgcc ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc
     1561 gtggcattat tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata
     1621 ccaccggcag ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga
     1681 gcctgattaa acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt
     1741 ggacgaccct tgtcagatca aaggatcttc
//
