LOCUS       sequence-464854-        1798 bp DNA     circular SYN 14-MAR-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Linker_SynTer-L10_PartType8-0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera 
            Xanthobacter and Roseixanthobacter
  JOURNAL   ACS Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806.
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806."
FEATURES             Location/Qualifiers
     source          1..1798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(651..678)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        776..867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             868..1728
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1732..1771
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgc atatgcccac taagagtttg tagaaacgca aaaaggccat
      661 ccgtcaggat ggccttctgc ttaatttgat ggttatcaga agagtgtaag cgggttgact
      721 accgatgaga ccagagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
      781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      841 cttcaataat attgaaaaag gaagagtatg agcattcagc attttcgtgt ggcgctgatt
      901 ccgttttttg cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg
      961 aaagatgcgg aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc
     1021 ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa
     1081 gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt
     1141 cgtattcatt atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg
     1201 accgatggca tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc
     1261 gcggcgaacc tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat
     1321 aacatgggcg atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt
     1381 ccgaacgatg aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg
     1441 ctgacgggtg agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg
     1501 gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat
     1561 aaaagcggtg cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt
     1621 aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt
     1681 aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt
     1741 aaaggctcct tttggagcct ttttttttgg acgacccttg tcagatcaaa ggatcttc
//
