LOCUS       sequence-465234-        5183 bp DNA     circular SYN 13-MAR-2025
DEFINITION  AiE0600m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster
            A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, 
            Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick 
            J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio 
            N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, 
            Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, 
            Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, 
            Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, 
            Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, 
            Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, 
            Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, 
            Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, 
            Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu 
            M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood 
            T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch
            K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan 
            B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244.
  PUBMED    38915722
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 13, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244."
FEATURES             Location/Qualifiers
     source          1..5183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1119..1128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1131..1151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2217..2328
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2446..2586
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2446..2575
                     /label=AAV2 ITR
     rep_origin      2661..3116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2748..2767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2958..2979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3133..3152)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3252..3274
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3312..3330)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3398..3502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3503..4363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3721..3740)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4534..5122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5023..5042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacagg acttcaggga aggccacgga gcttaagcaa
      181 gaacttgccc aaagtttaga aagaaagctc caagttacag aaccaagtgc ccgaccccag
      241 agtctttctt tgtctgacct gagggctttc tgttaaaatc cttatacagc agataagtaa
      301 gtcagcagtc ttagaggtgg gaagaggtct gtctgccagt catctggctc cctgcaaaca
      361 caggcatccc cacccctgaa caattttctt cataggaaag ttgtgaggga agtcacaggt
      421 gcagacaccg ccccgggggg ggaggggggg ctcagagttg ttccttcatt cagtcaccaa
      481 ttcagtcttc cagaatcaag gccctattga cagagggcta agccaccggg gcgctgcctt
      541 caaggtaaga ggcatagatc agtctcctga atgtagaggg atgaattccc tccttcacat
      601 aagtgaaagc gatcataggg aacggaggca tcattagggc gccctccttc tgacagggat
      661 gcagacaggc aggtgagggt ggaggggtgt ggcccagggc acattcagag ccaaactctg
      721 ttgagaacaa ggcaggtgtt tctgaaaacc tctctgattg cacccgtacc ctcccatcag
      781 gcccgaatcg cacacctctc tgcctctcct cagacgccgg cccacatggc ttttgaaaac
      841 ctggggagat tataccttcc agcttgcttt gctgagtctg aggaacctgg ctgccttttc
      901 tcttctgtta actgcaatca aactggtttt gagcaccagc cccaagatgt ctttgccagt
      961 ccacatccat cttccatgat gagctaaggt tccctatttg tacccttact ttctgggctg
     1021 agtcatgaca tctgggagag ctcgggctgg gcataaaagt cagggcagag ccatctattg
     1081 cttacatttg cttctgggat ccagatcttt cgaagctagc caccatggtg cccaagaaga
     1141 agaggaaagt ctccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg
     1201 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
     1261 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
     1321 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc
     1381 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc
     1441 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga
     1501 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg
     1561 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca
     1621 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg
     1681 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg
     1741 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta
     1801 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc
     1861 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca
     1921 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg
     1981 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaccg
     2041 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg
     2101 tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc
     2161 tgctcgaaga tggggactga ggcgcgccga attcaagctt ctcgagagat cttcgactgt
     2221 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2281 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2341 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2401 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     2461 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     2521 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     2581 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2641 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2701 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     2761 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2821 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     2881 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2941 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     3001 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     3061 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3121 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3181 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3241 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3301 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3361 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5161 ttgctggcct tttgctcaca tgt
//
