LOCUS       sequence-465685-       10196 bp DNA     circular SYN 19-MAR-2025
DEFINITION  Expresses PABPC1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen 
            Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung 
            K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen 
            M, Huang H, Weng H, Xiao G, Deng X, Chen J
  TITLE     YTHDF2 promotes ATP synthesis and immune evasion in B cell 
            malignancies.
  JOURNAL   Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 
            10.1016/j.cell.2024.11.007.
  PUBMED    39694037
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007."
FEATURES             Location/Qualifiers
     source          1..10196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2290..2355
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        4338..4549
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4562..4830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4859..4868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4889..5551
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             5621..5674
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5675..6274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5675..5694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6171..6191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6275..6863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6328..6348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6746..6757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6937..7170
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7242..7363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7279..7298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7333..7352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7403..7538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7571..7587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7571..7587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7584..7606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7595..7611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7619..7649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7664..7685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7802..7819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7973..8561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8732..9592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9355..9374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9593..9697)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9765..9783
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9821..9843)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9943..9962
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10156..10178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10170..10187
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10171..10187
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagaatgg attacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     2341 gatgacgatg acaaggctag cgaattcgaa tttaaatcgg atccgatgaa ccccagtgcc
     2401 cccagctacc ccatggcctc gctctacgtg ggggacctcc accccgacgt gaccgaggcg
     2461 atgctctacg agaagttcag cccggccggg cccatcctct ccatccgggt ctgcagggac
     2521 atgatcaccc gccgctcctt gggctacgcg tatgtgaact tccagcagcc ggcggacgcg
     2581 gagcgtgctt tggacaccat gaattttgat gttataaagg gcaagccagt acgcatcatg
     2641 tggtctcagc gtgatccatc acttcgcaaa agtggagtag gcaacatatt cattaaaaat
     2701 ctggacaaat ccattgataa taaagcactg tatgatacat tttctgcttt tggtaacatc
     2761 ctttcatgta aggtggtttg tgatgaaaat ggttccaagg gctatggatt tgtacacttt
     2821 gagacgcagg aagcagctga aagagctatt gaaaaaatga atggaatgct cctaaatgat
     2881 cgcaaagtat ttgttggacg atttaagtct cgtaaagaac gagaagctga acttggagct
     2941 agggcaaaag aattcaccaa tgtttacatc aagaattttg gagaagacat ggatgatgag
     3001 cgccttaagg atctctttgg caagtttggg cctgccttaa gtgtgaaagt aatgactgat
     3061 gaaagtggaa aatccaaagg atttggattt gtaagctttg aaaggcatga agatgcacag
     3121 aaagctgtgg atgagatgaa cggaaaggag ctcaatggaa aacaaattta tgttggtcga
     3181 gctcagaaaa aggtggaacg gcagacggaa cttaagcgca aatttgaaca gatgaaacaa
     3241 gataggatca ccagatacca gggtgttaat ctttatgtga aaaatcttga tgatggtatt
     3301 gatgatgaac gtctccggaa agagttttct ccatttggta caatcactag tgcaaaggtt
     3361 atgatggagg gtggtcgcag caaagggttt ggttttgtat gtttctcctc cccagaagaa
     3421 gccactaaag cagttacaga aatgaacggt agaattgtgg ccacaaagcc attgtatgta
     3481 gctttagctc agcgcaaaga agagcgccag gctcacctca ctaaccagta tatgcagaga
     3541 atggcaagtg tacgagctgt tcccaaccct gtaatcaacc cctaccagcc agcacctcct
     3601 tcaggttact tcatggcagc tatcccacag actcagaacc gtgctgcata ctatcctcct
     3661 agccaaattg ctcaactaag accaagtcct cgctggactg ctcagggtgc cagacctcat
     3721 ccattccaaa atatgcccgg tgctatccgc ccagctgctc ctagaccacc atttagtact
     3781 atgagaccag cttcttcaca ggttccacga gtcatgtcaa cacagcgtgt tgctaacaca
     3841 tcaacacaga caatgggtcc acgtcctgca gctgcagccg ctgcagctac tcctgctgtc
     3901 cgcaccgttc cacagtataa atatgctgca ggagttcgca atcctcagca acatcttaat
     3961 gcacagccac aagttacaat gcaacagcct gctgttcatg tacaaggtca ggaacctttg
     4021 actgcttcca tgttggcatc tgcccctcct caagagcaaa agcaaatgtt gggtgaacgg
     4081 ctgtttcctc ttattcaagc catgcaccct actcttgctg gtaaaatcac tggcatgttg
     4141 ttggagattg ataattcaga acttcttcat atgctcgagt ctccagagtc actccgttct
     4201 aaggttgatg aagctgtagc tgtactacaa gcccaccaag ctaaagaggc tgcccagaaa
     4261 gcagttaaca gtgccaccgg tgttccaact gtttaagcgg ccgcgaagga tctgcgatcg
     4321 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4381 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4441 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4501 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     4561 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     4621 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     4681 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     4741 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     4801 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     4861 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     4921 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     4981 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     5041 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     5101 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     5161 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     5221 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     5281 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     5341 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     5401 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     5461 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     5521 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     5581 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     5641 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     5701 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5761 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6241 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     6301 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6361 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6421 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6481 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6541 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6601 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6661 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6721 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6781 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     6841 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     6901 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6961 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7021 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7081 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7141 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7201 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7381 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat
     7561 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9721 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9781 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9841 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9901 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     9961 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10021 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10081 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10141 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
