LOCUS       sequence-466919-        6821 bp DNA     circular SYN 27-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal 
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
  PUBMED    18160654
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2007 Dec 26. 27(52):14459-69."
FEATURES             Location/Qualifiers
     source          1..6821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1990..2124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2014..2033)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2068..2087
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2130..2325)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2144..2279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2384..2405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2420..2450
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2458..2474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2463..2485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2482..2498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2482..2498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2841..2860
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2859..2914
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2960..2979
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4252..4272)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             4752..4763
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(5136..5155)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5163..6179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5217..5239)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6180..6436)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     enhancer        6438..6817
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagcgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2041 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2101 attataagct gcaataaaca agttagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     2161 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     2221 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     2281 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcggatc cgctggcacg
     2341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2461 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgggc
     2521 tgcaggtcga gggatctcca taagagaaga gggacagcta tgactgggag tagtcaggag
     2581 aggaggaaaa atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa
     2641 aataacacaa aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat
     2701 aaggaatgga cagcaggggg ctgtttcata tactgatgac ctctttatag ccaacctttg
     2761 ttcatggcag ccagcatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga
     2821 tgttttaaat gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa
     2881 cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc
     2941 ttcatgatgt ccccataatt tttggcagag ggaaaaagat ctctcgaggc ggccgcgata
     3001 tcctagttct gcatctgctc aaagaacttg taggttggat tttcgtagcc gttctgctgc
     3061 atcttggaca ggtggcgctc ctctggggtg acagcggcgt caacctccac cacaccatga
     3121 tgaatggatg tgtactgttt cttcttcagc atcaccaagg tgatgacgat cactgtcgct
     3181 atgacaacac cgcccaccat gagtccaatg attgcacctt tgtttgaacc cacatcttct
     3241 gcaaagaaca ccaatttttg atgatgaact tcatatcctg agtcatgtcg gaattctgca
     3301 tccatcttca cttcagagat ctcctccgtc ttgatatttg tcaacccaga acctggtcga
     3361 gtggtcagtc ctcggtcggc agcagggcgg gcatcaacag gctcaacttc gttttctgtg
     3421 ttggctggca cagagtcagc cccaaaagaa tgccacggct ggagatcgtc caggctgaac
     3481 tctccattca cgggaaggag ctccacggtg gttttcgttt cggtcaaaga tggcatgaga
     3541 gcatcgtttc cgtaactgat ccttggttca ctaatcatgt tggccaagac gtcatctgaa
     3601 tagttttgct ctttctgaag cagctcatca acttcatcct gaatctcctc ggccactgca
     3661 ggcacgttgt agagcaggga gagagactga ttcatgcgct cataaatcac acggaggtgt
     3721 gtcataacct gggaccggat ctgagcggct ttcttgggat ccaccatgcg cacatgctcg
     3781 aaatgcttta gggtgtgctg tctgtccttc tgttctgcgc ggacatactt ctttagcata
     3841 ttgaacacgt gacgaggccg aggaggaaca gcctgcagag cggtgatgta gttctccagg
     3901 gccaggcggc ggcggtcatt gagcatggct tccactctgg ccatgtgtgt ctccaccagc
     3961 tgctgtctct cgttggctgc ttcctgttcc aaagattcca ctttctcctg gaaatgctgg
     4021 ataactgcct tcttatcagc tttaggcaag ttctttgctt gacgttctgc ctcttcccat
     4081 tctctcatga cctgggacat tctctctcgg tgcttggcct caagcctctc tttggctttc
     4141 tggaaatggg catgttcatt ctcatcccca ggtgtctcga gatacttgtc aacggcatca
     4201 ggggtactgg ctgctgttgt aggaactcga accacctctt ccacagactc tgtggtggtg
     4261 gtggtggtgg tggcaatgct ggtggttctc tctgtggctt cttcgtaggg ttcctcagcc
     4321 tcttcctcta cctcatcacc atcctcatcg tcctcgtcat catcggcttc ttcttcttcc
     4381 acctcagcca cttcttcctc ctctgctact tctactactt tgtcttcact cccatctgca
     4441 tagtctgtgt ctgctccgcc ccaccagaca tccgagtcat cctcctccgc atcagcagaa
     4501 tccacattgt cactttcttc agccagtggg caacacacaa actctacccc tcggaacttg
     4561 tcaattccgc agggcagcaa catgccgtag tcatgcaagt tggtactctt ctcactgcat
     4621 gtctctttgg cgacggtgtg ccagtgaaga tgagtttcgc aaacatccat cctctcctgg
     4681 tgtaagaatt tgcacttgtc aggaacgaga agggcatcac ttacaaactc accaactaag
     4741 cagcggtagg gaatcacaaa gtggggatgg gtcttgcact gcttgcggcc ccgcttgcac
     4801 cagttctgga tggtcactgg ttggttggct tctaccacat tggtgatctg cagttcaggg
     4861 tagacttctt ggcaatactg caggatgcct tccttggtat caatgcaggt tttggtccct
     4921 gatggatctg aatcccactt cccattctgg acattcatgt gcatgttcag tctgccacag
     4981 aacatggcaa tctggggttc agccagcagg ccagcattac catcagtggg tacctccagc
     5041 gcccgagccg tccaggcggc cagcaggagc agtgccaaac cgggcagcat ggcaagctat
     5101 cggtacctga attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg
     5161 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc
     5221 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc
     5281 ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc
     5341 tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg
     5401 gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc
     5461 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
     5521 ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc
     5581 gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca
     5641 cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg
     5701 tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac
     5761 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc
     5821 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
     5881 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg
     5941 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt
     6001 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
     6061 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
     6121 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc
     6181 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
     6241 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
     6301 cgcccctcgc cccgcccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa
     6361 ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac
     6421 gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     6481 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     6541 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     6601 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6661 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6721 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     6781 attgattact attaataact agtcaataat caatgtcgac c
//
