LOCUS       sequence-467487-       20559 bp DNA     circular SYN 02-APR-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate 
            inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2025 from SnapGene Server 7.0.3
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
FEATURES             Location/Qualifiers
     source          1..20559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      4309..4318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5611..5628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      14183..14210
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14342..14369
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14801..15652)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15639..16478)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16989..17960)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19199..19286
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19628..20022
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20185..20228)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20324..20433
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg gaagacgacc gtgacaaggc gcgcgttcgc tcagcttgcc
     2641 gccgcgacag gcgcaatagc cgctatgggt gttggaactc gtcctgcggt ggcgcttacc
     2701 gatggcaatt cggggacggc gggcgagcgg gggatcaaga agatacgctc gtgctgccgc
     2761 ggctgcggaa aggtcgaatg cggtgtgtgg gtgtacatcc aagatggcaa agtggttcga
     2821 accgaaggtg acgaaacctg cttcaacact atgggcaatc attgcagcaa ggggcaagcg
     2881 tccattcagg ctgcatatca tcctgatcgt atcaagttcc caatgaagcg cacgaacccg
     2941 aagggcagcg aagatccagg ttgggtgagg atcagctggg acgaggccta ccggaccatt
     3001 gcggacaata ttatgcagtt gcgtgagaag tacggtcctg aaagcttgtt cacgtggtgc
     3061 ggtacgggcc gacagtggtg catgcagtcc gatgctggca tggcgcttga gctgttcggt
     3121 acgccgaaca tcatcgcggc gtaccaagtg tgcaagggtc cgcgccattt ttcgtcgcgt
     3181 ctcgacaacg ttcaggcgtg gtcttggagt gaggtgataa accattcgac gaaatacgtc
     3241 caatgggcta ccgatccctc cgtttcgaat tacgacgatt cgtctcgttt ggtaattgat
     3301 gtggcgcgcg aagctgaggc ttttatcgtg gttgaccctc gcctgtcgaa tcttggccgc
     3361 actgcgaagt attggttaaa cttgcgacct ggcaccgata atgccatggc gctaggatgg
     3421 tgccatatca tattgaagca cgatctggtg gattggcagt tcgtgaaacg ctggtccaat
     3481 gcctcgttca tcgtcgtgcc cgatatggac ccttcgggct acaccgaggc ggttcaaaac
     3541 acgaaaagcc cctacgaata tcgtacgcgt ttgctgaccg aagccgacat agatccttct
     3601 atggttgact gggagattga aggcgagggg aatccaaaac gttatcttgt gtacgatcag
     3661 atcaatcggc gctggacata ttggcaagcc gatcccgaag acgcgcattg ggaaggcgag
     3721 acctggacaa aacaaacctc tggattcacg caagacgttt cgcgtcttcg cgacgacgaa
     3781 tcgaaggtgg ctggctggat cgcggatctg tcggaattcg atccgcgtat cgatccggcg
     3841 ctcaccgggg aattcgaagt aaggcttaag gacggaagta cgcatatcgg ccgtcctgca
     3901 tgggatttgt gggctgagta cctgcaacaa ttcactcccg aacgagtgtc ggagatcacg
     3961 ggcgtagatg cccagcttat cgaagacgcc gtggttgaat gggcaacgcg cgatgacccg
     4021 cgcataccca acggcggtat aaattacggt ctgggcgtgg agcatgcggg caactcaacg
     4081 cagaattgca gggccattat ggcggcttgc gcaatggtag gcgccatcga cacgccgggc
     4141 ggccagcgcg gtgcgacgaa cggatggacc gaacagtcgg gtccgtgcgc catgcttccg
     4201 tcgatggcgg cattcgcgtt catgcccacg cccgacttgt cgctcaagat ggccggaaat
     4261 gagaagatgc cgcttctata ctggtacggt gtgtggtgcg atgccaatgc cgccatggaa
     4321 tgcgcgcaca aggagccgga tgcgccctat gagattcatg gtggcatgat cggctcgggc
     4381 gaccatatga acatgggcaa cgcaacgtac aattgggaag cgctcaacat gctcgacttt
     4441 ctgttcgagg cgaatctgtg gcattcccct acttcaggtg cggccgacat cctccttccg
     4501 gtctgccatt ggaccgagat caacgcgtat cgcattgccc aaggggcatc cggtgggttt
     4561 ggcctatgcg tgaaagctgt tgatcctcct ggcgaatgca aaagcgatcc gttgtacttc
     4621 atggagctgt cgaagtattt cggcgtccca gcgtttgacg gcgacgatcc gtggctcgag
     4681 aacaaacctg atgccgatct cgaaatcgaa aacctcacga ttcagtgctg tgtccaagga
     4741 tgcgcgccat acaacaattg gaacgagttg gaggctgcat tccaagagca cggttggtgg
     4801 aacatgaaac gcgagatccc ggaagactgg ggcacgtacc gtcgatacga ggtggggcag
     4861 gcgtatcggt tggctcccca tcagcaaccg gctcagctga acatcaataa accggggttc
     4921 cccacaccta cgatgaaaca tgagttttgg tgcacttcca tcgaatcgtt cttcccggag
     4981 ggggcggatg gccctgagct tgcacccggg ttcacttccg aagcgctgcc ttattatgca
     5041 gagcccgcac acggccctgt ggtcgatgcg gaaacgtaca aggagtatcc cattacctgc
     5101 atcactggac gacgcatacc ggtgtacttc cactccgagc accgacagct gccctggtgt
     5161 cgcgagcttt ggccggtgcc tcgcatggag atcaatcccg atacggctgc tgaacttgga
     5221 ctcgaacaag gagattgggc ctggatcgag agcccttggg gcaaggtgcg acagacggtc
     5281 gatctctatt acggcattaa gccgaacatg atcaacgccg agcatcaatg gtggtatccg
     5341 gagttggctc aagcggacaa aggatatgag ttgtcatgca tcaattgcat taccgatcgg
     5401 aagactcagg ataaatacaa tggatcgtcg aatgtgcgca cctatccagt gaaggtgtac
     5461 aaagccacgc ctgagaattc cccctttggg aatcctatcc cttgcggaaa cgatgggact
     5521 gagatcattc atgactcttc tgatcctcgc ctcaagctat gggaaatcgg cgctgctggc
     5581 atccatccgg atcacttcga gggcagcagc catcaccatc accatcacta ggagaggggg
     5641 aattatgacg cagtacgcta tcgtcaccga tctcgatcgt tgcgtaggtt gtcttgcctg
     5701 ttcggttgcc tgcaaggtag tcaacggcgt tccgacgggc tcgttttgga acaagacatt
     5761 gcgcattggc ccgaatcctc gagaaggcgg cagtgggcaa tggcccgatg tggagctgta
     5821 ttttctcaat gtgcagtgcc agcactgcga gaaccccgaa tgcgtgaagg tgtgcccgac
     5881 gggcgcctcg cacaagaccg aggacggcac cgtgcagatc gacaagtcga agtgcatcgg
     5941 ctgccagttc tgcgcgatgt cgtgcccgta cggggtgcgc tacctcaacg aggaggagcg
     6001 cgtcgtggag aagtgcacct tgtgcgagca gaagatcgct cagggcgaac ttcctcagtg
     6061 cgtgagtcaa tgctgcggta tggcacgctg gttcggcgat gcggacgaag gtatcgaagg
     6121 tttcgtcggg cctcgcggcg agatactggg cgagtatata tcgccattct cggatgatca
     6181 ggtgtatagc ctgccggatg tgggcaataa gccgtcgctc aggtacatct tgcgcgatat
     6241 gaaatggcaa ggaggcggtg aatgatggaa ctgcaatggc ctttgatttt gttcaccacg
     6301 ctggttgcgt ggagcgcggg gctgttcggg acgcaggcgc tgatggcggt gttcggcgtc
     6361 gggaagaagg cgcaggtgcc ggcttgggtt tgctcggccg tcttgctggc ggccggcggg
     6421 atcgcggtgt tcttccatct cgagcactgg gagcggatct tcaacgggtt cgggcatttg
     6481 acgagcggga tcacgcagga attgatcgcg atcgtggtct tggccgtggt ggctatcgtc
     6541 tacttggtgc tcatgcgcaa gtccgacgac ggcgccagcg tgccgaagtg gctggcatgg
     6601 gtctccgtcg ccttgtcggt cgtgctcgtg gccgtcatgg cgcacagcta cacgatggcc
     6661 gcccgccccg cctgggactc ggccctctgg atcctctacg tcctcggcaa cgcctgcgtg
     6721 ttgggccccg cgacgttcgc cctcctctcc gccttggctg cagggggacc ccgggaccag
     6781 cccgccgagc gcgcagccga cgccggggcc cccgcgggtc tcgcggccct cgccggcgcc
     6841 gccctcaacg ccctcgcggc cctcgccttc gccgccttcc tccagctctc cgccggctcc
     6901 ttcgccgacg tcggcctcta cttcgacccg acgcacccca cgaaggccat ggccgacgcc
     6961 gccgcgacgg tcgcctccca ggccccgctg ctgtggctgg gcgccgtggc cgtgggcgcg
     7021 atcgtgcccc tggccgccgc cttcctaggc aggcgcacgg ggaactggaa gctctgggtc
     7081 ccggtcgcga tagccgccgc cctggtcggc gccgtctgca tgcgcgtcgt cttctacaac
     7141 ctggggctca gcgtgttcat gttctactag actagtccgc tggttgccac gacgccctcg
     7201 aagtctcctt cgagggcgtt ctgtcgtttg caagaagggt ctggagaggc aaatcgcgct
     7261 cgaaggggct tcgaggccgg ggtaggtcgg gagggtggaa tccagatgtt tcacgtgaaa
     7321 catccgttct tttacggccg gtgtttcgcg tgaaacattt tgcggaaagg actccttccc
     7381 ttcccgtgcg ccaccccaac gccccgctcc tagcgctcgt acacctggtc gcagttgagc
     7441 agatccttca tggagtgcca gatctggtgc gggttgcccg gcgggttctt gagcggcttc
     7501 atcggatagc gctcctgcgc gcccgacagc agatcgacgg cgaacacttc gtcggacagc
     7561 tcgagcgcgc cttccgggca gatgtcggtg cagcagcggc acttcacgca atcgaccgga
     7621 cgatgcgtca cgccgatcga gccgtcctca cccgcgtact tcgcgatggc cccggtgggg
     7681 cagaacgtgg cgcacatctg gcacgacgag cacttctcgg gatcgatgat cacatggccc
     7741 cacaagcgcg tgtccatgag cacatcctgc ggctgcccca acgaatcgag cgcggccagc
     7801 aaacgttcgc ggcggtcggg aatgaagtga ggcagcgtgc cgtccttgcc caccttcacg
     7861 aacctcggct cccgccgctc ctccacaccc aatgcgtcct tgacggcgta gtcagccgtc
     7921 accgccgcaa cgttcttagc ctcgtccttc atgctggaga agaagctccg acggctcgcg
     7981 tcgtagccct cgtcgcccgc ccggcgcacg gatgagggaa gcttttccgc caggtcgatg
     8041 cgcatatcgt tgttccaggt tgcaagcagc gcgcttgccg tgtcgcacac aacccgcgcc
     8101 atctcgaaac ccgagacatg ctcgcattgc gcgcagcgcg cctgcacgag gctcacgcgg
     8161 cgcacgccca acgccgccag cgtgaccagc aggctctcct ccacgcgccc caggcacgtg
     8221 acgcccacca ccttctcggg gtcgtacaac ccgtcggccg cggcaagcag ctgatcgcag
     8281 gcgataacca cctgtccgtc ggccacgtca gccgccgcgc ggcatgcttg cagaagctcg
     8341 gcatcgttcg gacgatgcgc ttcaagcgcg caggtgggac acacggtggc gcaggttccg
     8401 cacccgatgc accgctcggg ttcgatgacc agctcgttgt cgtcatagga gatgcaaccg
     8461 ctcgtgcaca cgtcggcgca cttcatgcac gttgcgttgc gattgcgcac aatagcgcaa
     8521 cgaccctgat gaaccgtaat gtcggcgctc tgaagttgtt cgagcagacc gaggaaattc
     8581 gtcacgtgag ccatgacttc accctttctc gtctaccccc ttgcaccgca cagtataacc
     8641 actttcgccg tctcacgcga gaaaaaacgc gcttgacacc ttcgacaggc ctccctatca
     8701 tgtggccttg gggagtccgc cggcacgctg atcgcgtgcg aagcggacat tgtttgcaaa
     8761 agtgggaacc tcaggggtga ggtttcgcgg tgctgtgcgc cgtaagtgca aacaatgtct
     8821 gacgaggggc ggaacatgac tcacgattcc acgaaccagc atctttctct tgagcccgac
     8881 tacgacgggt tcgatccact tgaagatttc gaagacgatg cgaccgaggt cgaaggccgt
     8941 gccgacgagt tcgtcgccac ggtgagcgcc gccgaagacg cgctcgctca cgacgcgatg
     9001 gcacccgcca cgcccgccat ctgcgaaaag cccgctgaag agcgcatcgc cgacttgttg
     9061 gaggccatgg cgccgcgccg cacggtgctg ctgggcatcc tggccgcctg cgccgagccg
     9121 cagccggcat ccgccgtgaa cgctcgcgtc gacgagctgc aggagaacaa cttctcggtg
     9181 tacagcgcgg ccaacctgtg ctccttgctg gagaaggcgg gcgccatcga gcgcgtcacg
     9241 gccgagggcg aacccgcgga ggacatcgaa atcgagccac agaccgtcgt cgtcgacggc
     9301 gtcgaatacc tggaagcccg cgaacccgtc gagatctact ggcacatcac cgagccgggc
     9361 cgcgcggcgc ttgaggccga caaaccgctc gagcgcctgc gcgccctcct ggacgaagat
     9421 gccgcctacg cgcccatcta ccagcgcatc ctccgcttgt gcacagcaga cggcggcgcc
     9481 acgacacccg ccatcaacaa cgcggtcgac cacaacccgc tggtgcagaa gccgcgcttc
     9541 tacgcgcccc acttcgtcga ccgcctggag aaatgcgacg cgctggcctg gaagaaggcg
     9601 tggtgcatca ccgacatcgg acgcgccggc ctcgatatgc tggcagacgt cgtagacgag
     9661 aacgcgcccg ccacccagcc cgaaaccccc gcaacccctg atcccgccgc atccaaggag
     9721 gactaaccat gaccgaagca accgcgcccg caaccgagac cctcgccgac gaagcgacca
     9781 tgcaggagct cgtcaaactg aacgagcagc gcgccgccac gtacggcctg ctgtcgcgcc
     9841 tgtaccgcgt ggaggtggac cagccccttc tcgacgagct gcgcagcatg cgcttccccg
     9901 ccaagacggg caacgagaac gtggacgagg gctaccgcct gctggccacg tacctgagca
     9961 acacctggga caacagcgtc accgacctcg ccatcgacta cgtgcgcgtg ttcatcggac
    10021 acggcatcga cgcgttctcg gccgcctacc cgttcgaatc ggtgtacacc tccgagaagc
    10081 gcctgctcat gcaggaagca cgcgacgagg tgctggccat ctaccgcagc gcaggcctcg
    10141 acaagcagga cacatggaaa gagggcgagg atcatatcgc gctcgagctg gaattcgagc
    10201 agatcatggc cacgcgcacg gtcgaagcgc tgcgcaaagg cgacgaagac gaggccgtcg
    10261 cgctgctgac cacgcagaag aactttctcg aagaccatct ggcggcatgg gctcccatga
    10321 tgacctccga catgaagcga ttcgcgcaaa ccgacttcta ccggggcttg gcgtacttga
    10381 cggacgggtt cctccaaacc gaccaaacgt tcctcgacga cgtgctggcc gaagactaac
    10441 tgcacaggat cgaaagaagg agcgatggac aacaagcagg agaaaaccta cgccggcatg
    10501 acgcgacgca cgctgtgcct gggcgcaggc ggcgctgcgg tcatgctagg tctaggcgga
    10561 ttgaagtacg cgggatcgca accgctcgtg cgcccgccgg gaggccagga cgaggaacgg
    10621 ctcatctcgg cctgcatccg ctgcgagaag tgctacgaga tctgcccgcg cgacgtcatc
    10681 gcgcccgccc acctcgagga cggtgtgctg aacatgcgca cccccacgtt cgacttctcg
    10741 gccaactggt gcgactggtg cgccgaagag cacgacggca ccccgttgtg cgtgagcgcc
    10801 tgccccaccg aagcgctcaa gctgcccgac gacgccacgg ccgagaacac catcatcggc
    10861 aaagccgtta tcaacacgga ctggtgcttg gcgtacaagc tgatcggatg ccggttttgc
    10921 tacgacgcct gtccctacga ggccatggag ctcgacgaga acaaccgccc cgtcgtgatc
    10981 gaggacaagt gcaacggctg cggcgcgtgc gagagcgtgt gcgtctcgct gcagaacggc
    11041 tccatctccg aaggagccac cgcgcgcgcc atcgtcgtcc acccgcttga cgaagttgaa
    11101 ggataaggtg ccctcatgaa gaagaactcc aagaagctgc gcaccctcgt cgcactggcc
    11161 atcgtggccg tcgtcgccgt cggcttcgtc acgaacgtcg ggatcggcac catgtcggca
    11221 ccgggcatct gggacatctc catcctatgc ccgctgggcg ccttgggaac gatgctcgcc
    11281 tccaagatga tggtgccgcg cgccgtaatc tcgctgatcg tgatggtgct gctcatcgtt
    11341 ctgttcgccc gcgcgttctg cggatggatt tgccccgtgc cgctcgtgca gaagttgcgc
    11401 ggcttgttcg cgaagaagca acccgtcgaa cgcggcgacg atgccgatcc tgccgcggcg
    11461 ccgctcacga aagtcgagat cgacgcgctg ggcgtttcat gctcgaaggg gtcgaagaat
    11521 ggcagcggat gcgcgtcctg cgcctccaag cgcggagccg cgctcgatgc tcgccacttc
    11581 gtgctgggcg gcgcgctggc gtccaccttc atctttggat tcccggtgtt ctgcctcgtc
    11641 tgccccatcg gactcacgtt cgccaccatc ctgctggtca tcaacctgtt ctcacacggc
    11701 gatgtcacct ggtcgctcat cgtggtgccc gcgctgctta tcgccgaggt gctgctgttc
    11761 aagaagtggt gccataagct gtgcccgctt tcggcgttca tgagcctgat cgcgaaggcg
    11821 aacaagacct tcgtgcccac gatcgacgat gcgaagtgcc tggaaacggc gaagggcggc
    11881 gcgtgcgggg cctgcggccg cgtctgcgag gagggcatcg acccgcgcca cccgcagctt
    11941 tcgcaggcgg catggagcga gtgcaccaag tgccgcgcct gcgtggacgc atgccctgcg
    12001 aacgcgatca ccatgccgct gctgccgcgg cgaacgccgt ctaccgcaga acctgtcgcc
    12061 gtgccaacga acgccgagaa gatcgacgcg gagaatgcgc gccccgttcc gtaaagcccc
    12121 tcctgcgcga cccggctcgc agctgcagcg gtctggtcgc gcatccggaa tcgggccgag
    12181 cccgaccccg ggcaaaaggc ggcttggcaa gggctttggg ggacgagccg ccttttgccc
    12241 gggagcgaca tccccgctcc ccctccctgc gaaaagaggg atgccgcttc cggccttcat
    12301 ctgacaacca cgacgtggga ggacgaaaac ccatggcata cgaaacgaca agcgaatcga
    12361 ccgaggccct gctcgtcacc ggaggcgtcg gcaccggcaa aacgcaacgt ctcgtcgagc
    12421 gcgctgccgc gctgctggcc gaaaacgccg ccgcgggcaa cgtgctcgtg ctgtgcgcga
    12481 cgccccaggc cgcacgcctg ttctcggagc ggttgacagc ccgcgtcggt gccaagcgcg
    12541 ctgccggcgc aaccgtcgcc accgcacgcg ccttagcgct cgacgtgctg gccgacgacg
    12601 gcgcgcgccg ctggtcgggg cgcgagccgc gactactgac cgccttcgaa gagacgttcc
    12661 tcatggagga catgaaggtg agcgggctgc ggccgaagcg gctgcgcgag atgctcaagt
    12721 tcttctaccg ctcgtggacc gagctggcgg acgacgatcc cgattggctg ctaccgggcg
    12781 aggaggccga tgtccatacg ctgttgaaag agaacctggt cttcatgcgc gccgtcatag
    12841 aacccgaagc ggcgaacctt gctgcacgct acctccgaga acacgacgaa gcgcgggcgg
    12901 cgcattccta cgcgcacgtg ctgatggacg actacgcctg cgcaagccgt gcctcgcaga
    12961 cgctggcgaa tctcgtcgca acctgctcga tcaccgtggc gggcgacgcg gaaacctgca
    13021 tcgagacata cgattcgtac ccctatgccg ccggaatcga cgaattcctc gcaacgcatc
    13081 ccgatgctgc ttgcgagcac ctggatgccg acgcgcccga tgccgtcgaa cgggtcgctc
    13141 tggcaagcga cacgcccgaa gccgagttcg aacaggtggc gcgcacggtg gccgaagccg
    13201 tcgccgcagg aacgcccgca cggcagatcg tcgtggccgt accgaacagc gtgtggtcgc
    13261 gcaacatcgc aaccgctttg cacgcacgag gcgtgccaac cgaagcgctc gccatctcgc
    13321 agcccctgcg cggcgacgtg cgggacaacg cccgctgcac gcctgcgcgc atcgccacgg
    13381 ccctcaacct cgtggcgaac ccagacgaca ccgcggcttg gcggtgctgg tgtggattcg
    13441 gcgactacct ggtgaacagc gcggctttcg ccagcctgcg gacttgcgcg aaggagcgca
    13501 gtatcggcct cgtggacgct ttggagatgc taagcgaaaa cgactgcgag cacgtagtgg
    13561 gcgcgcaacg cgtggcgacc gcctacaagg acggtcgcgc gctcatcgag caggtacgag
    13621 gattgcgggg cacggcactg ctggacgagc tgacgcgcat cgtcaccgac ggagcggaat
    13681 cccaagcgcc cgccgttctg cgcgagctct gcctcgagca cggcaacgca gccgatgaaa
    13741 acccggaaag cctttacgat gccgatgctg cggcgatggc gcggcgcatg gccgagcgcc
    13801 tgctcgcgcc cacgctggag gttgccgacg cggtagccgt cgtcccctac gacttcgcct
    13861 gcggcttctc gcccgacctg ctgatcgtcg ccggcttcgt gaacggtttc atcccctccc
    13921 gcgactattt cgacgccacg gtgaccccgc ccgagcgcca ggagaagatg cgcgccgtcg
    13981 acgaacgacg cgtgcgcgcg ctcacaggaa aggccggccg cgcggtggcg ctgtcgtact
    14041 tcacgtccac cgacctcgaa agcgccggca agctgaagct caagatcagt cgcatccgcc
    14101 tcaagaacgg tatccgcacc tgcactatag agcccagcct attcctggag caaatcgcag
    14161 cgcaggagta gcgactcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
    14221 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt aattcctgtt
    14281 ataaaaaaag gatcaatttt gaactctctc ccaaagttga tcccttaacg atccctgcag
    14341 gagaaggcca tcctgacgga tggccttttt gaagctttct agaatcgggg gcccccgggg
    14401 ggattctaga acaatggaag tcctcgtctc gccctcgaat tagcccgctt aatgagcggg
    14461 ctttttttga attaattctc gcgagctggc acgacaggtt tcccgactgg aaagcgggca
    14521 gtgagcgcaa cgcaattaat gtaagttagc gcgaattgca agctggccga cgcgctgggc
    14581 tacgtcttgc tggcgttcgg gagcagaaga gcatacatct ggaagcaaag ccaggaaagc
    14641 ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt
    14701 ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat
    14761 aaaagataaa aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc
    14821 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct
    14881 ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg
    14941 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga
    15001 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct
    15061 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt
    15121 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa
    15181 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc
    15241 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca
    15301 gcgcccgata gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt
    15361 ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc
    15421 cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg
    15481 gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct
    15541 tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg
    15601 ccgggtcgtg tcggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc
    15661 cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc
    15721 ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg
    15781 aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc
    15841 gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc
    15901 tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata
    15961 gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg
    16021 ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg
    16081 ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg
    16141 ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg
    16201 tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg
    16261 tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc
    16321 tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc
    16381 ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg
    16441 aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag
    16501 ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca
    16561 ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc
    16621 ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca
    16681 ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg
    16741 ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc
    16801 atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg
    16861 ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg
    16921 tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa
    16981 cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc
    17041 ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg
    17101 acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg
    17161 gcctcggcca tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc
    17221 gcggcgataa agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc
    17281 tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcggacagt
    17341 tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg
    17401 ccagcctgct gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc
    17461 agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc
    17521 gccaagcggc catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc
    17581 agcgtccggg caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat
    17641 gcctgatagt tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc
    17701 atgtcatcca ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc
    17761 tcggcgggcc tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg
    17821 ttctggagca cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc
    17881 ctgacgccga tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc
    17941 aaagtcctgt cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg
    18001 tcagtggcgt caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca
    18061 tggaagccag catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg
    18121 cgctctgcgc ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca
    18181 cccatgccgc ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg
    18241 cctgctgggt ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg
    18301 ccagcggttc gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga
    18361 ttgcgttcat ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg
    18421 gtgtcgatgt tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc
    18481 accacgttcg gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt
    18541 ctgtggtcaa tgcgggcgtc gtggcctgcc cgctctaatg cccggttggc atggtcggcc
    18601 catgcctcgc gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc
    18661 ccgcccttct ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg
    18721 atgccgtcat tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg
    18781 gcatggatgg ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg
    18841 gacgccagcg ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc
    18901 ttgaacagcc gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc
    18961 cgctcgacga actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg
    19021 gcatacttgc cttcgcgctg gatgaagtcg gccttggccc tggccgattg gccgcccgac
    19081 ctgctgccgg ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc
    19141 ttttcggctc catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc
    19201 agtttctcga agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc
    19261 gccgctgtgc ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg
    19321 ttaaggggag caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa
    19381 tcaatgccga aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa
    19441 caaggcgcaa ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc
    19501 cggaggatcg gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct
    19561 tgttcggtct gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc
    19621 cctgcggggc tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc
    19681 taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc
    19741 ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc
    19801 tatccggcct ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat
    19861 aaccgcgcct agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca
    19921 gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg
    19981 acagttattg caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag
    20041 tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa
    20101 ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct
    20161 catgtggcgg ccctcgcgag aattaattca gataaaaaaa atccttagct ttcgctaagg
    20221 atgatttcta gcgatgaccc tgctgattgg ttcgctgacc gtagactggt tggcttggtt
    20281 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
    20341 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
    20401 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
    20461 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgacatcgcg cgcgcgcgcg
    20521 cggatgctag cggcgcgcca aaaaaaagcc cgctcatta
//
