LOCUS       sequence-100130-         740 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange 
            dsRed with flip-out conditional cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-1-FRT-2xTY1-FRT-V5
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..98
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    267..314
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    380..427
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             429..470
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    656..725
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 ggagctcgct agcgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
       61 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atggtcgact
      121 ctagaagtcg atccaacatg gcgacttgtc ccatccccgg catgtttaaa tatactaatt
      181 attcttgaac taattttaat caaccgattt atctctcttc cgcaggtggc ggaggttccg
      241 gtggaagcgg aggtagcggc ggatcggaag ttcctattcc gaagttccta ttctctagaa
      301 agtataggaa cttcgaggtc cacactaatc aagaccccct ggatgccgag gtgcacacca
      361 accaggaccc tctggaccgg aagttcctat tccgaagttc ctattctcta gaaagtatag
      421 gaacttcggg caagcccatc cccaaccccc tgctgggcct ggatagcacc agatccggag
      481 gtagcggtgg aagcggaggt tccggaggta agttattgaa caatggcatc aaatgccttc
      541 atcatcacta ccctttagcc cttaagaccc cacaatgacc ttacccactc agagaaaaaa
      601 gtaaatatga aagcccattt gaacttctaa gcttgtcgac catcatgatg gaccagatgg
      661 gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg caccgcggct
      721 tcgagaccgt gacctacatc
//
