LOCUS       sequence-100273-         843 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Contains a promoter of two gal binding sites coupled with a core 
            promoter derived from native Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS13-Pleum
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    558..675
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(736..756)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(736..754)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(775..791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(775..791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(788..810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    799..815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acatcaccat ctaattcacc aaaattggga caacaccagt gaataattct tcacctttag
       61 acatttttct agattagtgt gtgtatttgt gtttgcgtgt ctatagaagt atagtaattt
      121 atgctgcaaa ggtcctaatg tataaggaaa gaatatttag agaaaagaag aaaacaagag
      181 ttttatatac atacagagca catgcatgcc atatgatcat gtgtcgtcgc acacatatat
      241 atatgcctgt atgtgtcagc actaaagttg cctggccatc cacgctatat atacacgcct
      301 ggcggatctg ctcgaggatt gcctacgcgt gggcttgatc caccaaccaa cgctcgccaa
      361 atgtttaaac ctgttaatag atcaaaaatc atcgcttcgc tgattaatta ccccagaaat
      421 aaggctaaaa aactaatcgc attatcatcc tatggttgtt aatttgattc gttaatttga
      481 aggtttgtgg ggccaggtta ctgccaattt ttcctcttca taaccataaa agctagtatt
      541 gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg
      601 accggtgaag acgaggacgc acggaggaga gtcttccgtc ggagggctgt cgcccgctcg
      661 gcggcttcta atccgtacta aagcttccgt taattaaggg gcgcgccaag gatcccggag
      721 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      841 cat
//
