LOCUS       sequence-100280-         947 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  to express human Pol iota recoded to yeast codons in yeast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Makarova AV, Grabow C, Gening LV, Tarantul VZ, Tahirov TH, Bessho T,
            Pavlov YI
  TITLE     Inaccurate DNA synthesis in cell extracts of yeast producing active 
            human DNA polymerase iota.
  JOURNAL   PLoS One. 2011 Jan 31;6(1):e16612. doi: 
            10.1371/journal.pone.0016612.
  PUBMED    21304950
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-01-31"; volume: "6(1):e16612. doi"; pages: " 
            10.1371/journal.pone.0016612"
COMMENT     Sequence Label: pESC-URA-hPOLISc
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             39..68
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtaatacga ctcactatag ggcccgggcg tcgacatgga acagaagttg atttccgaag
       61 aagacctcga gatggaattg gctgacgttg gtgctgctgc ttcttctcaa ggtgttcacg
      121 accaagtttt gccaacccca aacgcttctt ctagagttat cgttcacgtt gacttggact
      181 gtttctacgc tcaagttgaa atgatctcta acccagaatt gaaggacaag ccattgggtg
      241 ttcaacaaaa gtacttggtt gttacctgta actacgaagc tagaaagttg ggtgttaaga
      301 agttgatgaa cgttagagac gctaaggaaa agtgtccaca attggttttg gttaacggtg
      361 aagacttgac cagatacaga gaaatgtctt acaaggttac cgaattgttg gaagaattct
      421 ctccagttgt tgaaagattg ggtttcgacg aaaacttcgt tgacttgacc gaaatggttg
      481 aaaagagatt gcaacaattg caatctgacg aattgtctgc tgttaccgtt tctggtcacg
      541 tttacaacaa ccaatctatc aacttgttgg acgttttgca catcagattg ttggttggtt
      601 ctcaaatcgc tgctgaaatg agagaagcta tgtacaacca attgggtttg accggttgtg
      661 ctggtgttgc ttctaacaag ttgttggcta agttggtttc tggtgttttc aagccaaacc
      721 aacaaaccgt tttgttgcca gaatcttgtc aacacttgat ccactctttg aaccacatca
      781 aggaaatccc aggtatcggt tacaagaccg ctaagtgttt ggaagctttg ggtatcaact
      841 ctgttagaga cttgcaaacc ttctctccaa agatcttgga aaaggaattg ggtatctctg
      901 ttgctcaaag aatccaaaag ttgtctttcg gtgaagacaa ctctcca
//
