LOCUS       sequence-100358-       18606 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Target a Cre-dependent GFP expression cassette to the stop codon of 
            the mouse Snap25 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18606)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 18606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Snap25-LSL-2A-GFP targeting vector
FEATURES             Location/Qualifiers
     source          1..18606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7274..7307
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7333..7794
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    7801..7834
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7864..7929
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(7930..7932,7933..8646)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7930..7932,7933..8643)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7972..7993)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8233..8252)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8580..8601
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8681..9269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8734..8754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9152..9163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9282..9299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9288..9495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9549..9582
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9609..10108
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9644..9667)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10041..10060
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        10125..10172
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10191..10994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10254..10273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10864..10883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11004..11465
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    12259..12380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12296..12315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12350..12369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(16418..16438)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16418..16436)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16457..16473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16457..16473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16470..16492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16481..16497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16505..16511,16512..16529,16530..16535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16550..16571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16688..16705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16859..17447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16939..16958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17618..18409,18410..18478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18241..18260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18479..18583)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 cgccggcgtt atccagaagc gatcgcaggt cagcttctca gggcacagag caggggaagt
      721 caaagacctg atttagagta ggaaaaggag gaaaaagtgg aatacacatt aaccacttct
      781 tttgagatct agaagcataa ataaataaat aaataaataa ataaataaat acctatttta
      841 aaagctcaaa ttatatgtca gccaatatct tggcctgagg aaaggaaacc actaagcaga
      901 aaaacagcca aatattttct atgacaaagg aaagattaaa gttattatat ttttgggcat
      961 gctcccccca attcattcca tacaaccacc agtttgtttt aaccagaaaa gcgtaatttt
     1021 gtcaaagaaa gtgtaagcct tatcaattaa aacagtttag acttattaca tatcaacaaa
     1081 cagttctcaa cctgtgagtt ataactgctt tagcaaacct ctgtctccag aaataattac
     1141 agtacaattg aaaacagtag caaaattgta cttatgatgt agcaacaaaa ataattttct
     1201 attacatgag aatctgcatt aaaggattgc agcatgaagc aggctgagaa tcaatgatat
     1261 acactgttat accagaactc agcataggtg aagatatttg tcttcctaat tatttatctc
     1321 taagttattg tagttattgg ccaatcaatt atgctggtat tcaaatgtga gccataagtc
     1381 atagggtcta gatatgcctt acaaatgaca attctaattt tgcctccatc tgcttacaag
     1441 gaaactcaat taataatttg taagccagtg gttgtaaaag tatggactat gggagatccg
     1501 aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaagg
     1561 aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg
     1621 aaggaagggg gaagcaggga aaaatgcaag aaggaaagac agagggacac gcgccaatat
     1681 ttaatccaca gcaattcaat ccaaatatct tgacaactga cacaagcatc tctgatttgt
     1741 acagctctgg tgttccactg tgctttaggg atggaagtag ttgtggtaca gaaacttgtt
     1801 catggttgat gatcactcaa atgaaatgta aatgatagga ggttttctct taatctttaa
     1861 atttgctctg tctcaggctg atatatatct tagtggagcc aagcatagtg gcataggact
     1921 ctaatcccag cactcatcag gtctgagttc tcaaagtcag cttgggctat atatggagac
     1981 aaggttgcaa gcaaaacaaa acaaacaata gacaaaagtt ataatatgcc agtgtacttt
     2041 gggagttgcc atgggaaaag gagtttaaac cttccgtttt tagctcatta ctgtaactag
     2101 taagaaaatt ctgtaataag tgctttctat ttgcagtaag atgcctgctg tttgcaatag
     2161 agtgccttct tagccttcaa gttggccttt ggaaacgtgg taaagtgctt tgtgagccag
     2221 ctcccgccct ccattcctac ccatacaagt gaaaccacca gattcacgtt tttatgctcc
     2281 aaaagcacct gttccaatgc ccctaccaaa cctatgacat caacatgtgt gtgtataaga
     2341 atcaccatga aaaagcagtt cctttaaatc tcttaggagt tctcaaaatc aagtgtataa
     2401 ctgctgtcta cattgccaca gaccccaagg tgggagagca atgagcactg agatttgcta
     2461 cctgactttc ttcactcatc ctcaagttga atcttggaaa tctatttttc agacaaacaa
     2521 agcaaaacaa aacaaaaata aaccatagtc tctatataat tttgcctcca tcctctgccc
     2581 tagagttcct cagaatagat gattttatct tccccaaaat aaattagggt atgagatggc
     2641 tgaggaatgt ctcagcaata aaacacttgc tgttcagggg agttcatatg cccaaaagcc
     2701 atgttaatgc cagttgtgat agtgtacacc taacattctt gtgtacctgt ggtgacatgg
     2761 gaagtgggaa ggataagccc actcatgaca tgtgtggtga tgagcaagac accctggtag
     2821 acatgataga gagcaatgac ttatacctag ctgctctctg accttcacat gggtcagatg
     2881 ccaggacaca cacacacaaa tatacattaa aaataagtaa aataataact aagaaggtga
     2941 aaattacata attttagaaa tcataaaagt aaatacatca gtgtgtttaa tatgaactat
     3001 aaattcaaag cagatgcaga gtgcatattt gtaatactaa cactgggaaa gatgagaagg
     3061 gtgaatcaag agttcagcca gccttggctt tatagccaaa ccctattgga gaaagataca
     3121 gcgagagaac tcaaagctcc caaatacagt atttgtatta gtacagaagt attctacctt
     3181 taaactacac tttgagctca aaacatcagt tttaaaattt cacttgaaat tgacaaatgt
     3241 tgttaaaaca tgttggcgta ttgaataaga attaggacta ttactagctg tgtgtattct
     3301 tcccacaagc tctacccctt ggtaacaact ttcttcttgc tctgtcttcc aaaattgctc
     3361 tttggctccc agggtaacaa atgatgcccg ggaaaatgag atggatgaga acctggagca
     3421 ggtgagcggc atcatcggaa acctccgcca tatggctcta gacatgggca atgagattga
     3481 cacccagaat cgccagatcg acaggatcat ggagaaggtg agtctacagt tctcaggtgc
     3541 ttactactta ttgccacgac atccacgcaa tcagtagttg ctggatactt tgcccttcat
     3601 atttcaaaac aaaaagcatg agactcagca gcagaactgg ggtgaagatt aaaatgcagt
     3661 ttgggtttca atggattgtg agtgctgggt attgagcaca gtgccatggg tatgctagaa
     3721 gactgttcca ccaatgagtt acactcctac cccaggccat acgactgaat gtagttgata
     3781 tttctctatc tctcccatgc caggcatgtg ctaggcactt agaacacatg aaaatgagac
     3841 agaatatatt ttcgctgcca aggaatttgt atcaggacac agaaaccatg aaacaattca
     3901 ccatacaaat cagacactaa ttctttcctt gcattctcat ccccaatcgc atgaatatta
     3961 attataatct ttttacgtgt ccaaatgcca agaggagaga tgtgtgcatc aagagtttaa
     4021 caatgatttt atgtgaatat atgtgagacc ctgaagttct taatctgaga ttttggagtc
     4081 tctttttatc ttttccattg ccaagaatag agtgggttct tttttacatc ttttgaatta
     4141 caaagctaag taatagtttc tggatctcca agcattcttc agtgccgtga agggatgaag
     4201 agacaggatt gtgtcacata tggtccagat aaacgatata tgaccacata atattttgcc
     4261 tgattggtat tgaactacac atatgtcaga gctggctaga aaaatgcttg tgttttgctt
     4321 aagtttgcaa gttacaacat gcttgtcatg ggtaagcagc ttttgctctc ataaagcttt
     4381 taatgctgtg tgagccaatg gattttagaa ccatgttgaa aggttcaaaa agtgttggaa
     4441 ccaattcagc ctgacctaag ctgttacagc tctaaaggaa aaatgaacag agaaataatt
     4501 tctccgctgt tttccagaaa ggaaacaatc tgagaaaaca ggaagtgctt gttttctatc
     4561 actgaaccaa attgactcag aagcaaggag ataactcaac aaagccaagc agggttccag
     4621 agaagagatt tttttttttt ttttctaact taagaggtca aaatgttgag aaactgtctt
     4681 ggaaaaagaa ttttttatcc tcttgtaaag acagcagaag ccacgttcta atacctatgc
     4741 cagtataatt ctctagaacg gcgagaactt tctgtattta gctgttcaac taccaataaa
     4801 ggtgtacaca gggataaaag tttaaggaaa ggctagacaa gttgatggga agcagctgct
     4861 ctggcttgct taaaccctag actgctgagt cactgagata gagctaaagg gtataattgc
     4921 cttacaataa ttagcatatc ttggctgata tttgcaatag aatcccacac acaaagtcac
     4981 aattggacac catttaaaca gcccatttga gtctggctga tcatgttact gagctacagt
     5041 attccctctt tctcaaggaa gttcttgtct ttgagaactg taccagaagt ctcagtatgt
     5101 ataaggttga gttaaaaccg cacagtaggc aaagcaaaga gaattttctt ctctcctggt
     5161 cccactatga tcttctctcg tcatcatttg ggttgagaga gaattctggg aagttcaaaa
     5221 cagagttttg atatagtctt ctggattttt aagtcattgc tttgtgggtt tgcttatgtg
     5281 tgtttatatg tacaaactat caaagttaca agcacattgt tcttctagaa tacttgaaat
     5341 tagaagtaag tttccatccc aagcttcata ctggtgctaa tgaaacaata aagatattca
     5401 aactccaaag taaggtttgg tgaaagaaaa tgtgctgtga tccagcgatg tggtcctcaa
     5461 ggtcttccac cagtggaggc cactgagctt gagaaataag acaacagctt agaaatggga
     5521 tttgtagttc accagctcta tgaactgggg aacattttaa ctctggatgt gctggtgtat
     5581 gacatagaca cagttgtaga tgtgtgatgt gggccagtgc agcacctgcc tgaagctaag
     5641 ggctcaatgt actcagtatc cccttctaac aaagtctggg ctccctgatg gacagtagcc
     5701 tttccacaaa ggcaacttag gaagcacgtc tcagaaatgt cagcaatctt aggactctct
     5761 aaaatcaagc aggaatctga ggatggggta tcctccattt aaaaacaaaa tcctcgagga
     5821 ttaaccagga aaacgggatg aacactgaca gaggcatttt ccaaaggaca tgcctgaggg
     5881 gagattctgt caggttttgt tttgttttgc ttttgttttc tatttctttt atataccaca
     5941 taaaaagtat gtggtacttt tgtgccatgt aatatgggtc tggaatttga atgtgactca
     6001 gagaacttga gaggaaggag caagcatcta ttgaacaaag gcattacagc tccaccctgt
     6061 cctttgagac tatcccaaga atagcctctg ctctacttac ccaagctgcc ttgcaaaggc
     6121 caggcagtgc attcataagc aagacagtct ccatttacct tcggaactga cgtttgtttt
     6181 aaaatggatt attttcttat ttaagcttct ctgagctcag tctagaaatg gcacaattca
     6241 gtctatagga gcatgtcaga ttggtctgag caaagtcatt ttccttttca acaaagtagg
     6301 attatttatg acacagttta taggtacaga agaaggatct gggaaccaaa taaaattgga
     6361 ggaattatcc atttttcaag tgagaagcct ggggggtcaa aaaatatgtg tctatagttc
     6421 tgaataatca ggacaaatca atgttctttt ttatgtaaat actcattttg aagccaactc
     6481 ttgattaacc aaacagatgg cagagaacca tagccagata attcagttgt gaataatcca
     6541 gaaatatgtt caactttcaa gatctttgtt tgaacataaa tgagtccagt gtgctaggaa
     6601 tttataaatt caaattgaat atagaaaact ctacccagtt gagattggaa agtgggaaag
     6661 taggcacata gcctatggtc agtgagtgat agtagatcat tccctcgacc acctggtggc
     6721 tttcttctct gcccaggctg tagagttaca agtgggagca ttgtttgtac agcacttgta
     6781 atgggagtcg acctctagaa gtaagctttg agctcaggaa ttcttggatc ctgtgcgaag
     6841 ttcctattct tcaaatagta taggaacttc tttcggccgg ccaaaagcaa cagcctgtac
     6901 ttgctgcctt acctttctcc atggcgatgg gaacagttaa tttcctaggc cttgacagtc
     6961 tagcctttga gcaccttccc agcataaaag ataaactata gaatactgtc ctcaaacagc
     7021 agatgctatc cagtgaggaa aaccattgaa acctcaagtc aagttcctgt gaccttgtcc
     7081 aatggtcttg ggggtggggg agtgatattt ctgaatgagt tgtttcacaa aagagcttga
     7141 gtggaccccg gggatgaata ggaccttaag gagtcgtcta agacctctct tctctcctct
     7201 tttctaggct gattccaaca aaaccagaat tgatgaagcc aaccaacgtg caacaaagat
     7261 gctgggaagt ggtataactt cgtataatgt atgctatacg aagttatcag gctgattaac
     7321 tagggggatc cgctgtaagt ctgcagaaat tgatgatcta ttaaacaata aagatgtcca
     7381 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
     7441 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct
     7501 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
     7561 aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag
     7621 atctatagat ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag
     7681 tactgtggtt tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt
     7741 tccacataca cttcattctc agtattgttt tgccaagttc taattccatc agaaagcttc
     7801 ataacttcgt ataatgtatg ctatacgaag ttatcacggg ctaagagagg ttctggagca
     7861 ccggtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt ggagtccaac
     7921 ccagggcccg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     7981 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     8041 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     8101 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     8161 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     8221 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     8281 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     8341 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     8401 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     8461 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     8521 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     8581 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     8641 aagtaacctc agcttctaga aatcacgtga gcttatcgat aatcaacctc tggattacaa
     8701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8761 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8821 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8881 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8941 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     9001 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     9061 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     9121 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     9181 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     9241 tcggatctcc ctttgggccg cctccccgca tcgataccgt gcctcgactg tgccttctag
     9301 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     9361 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     9421 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     9481 caggcatgct ggggaggcgc gccgtaaaag cttggcatag atcttattgc agaagccata
     9541 tgttttaggt gccagggcgt gcccttgggc tccccgggcg cgtttggatg catttatcga
     9601 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     9661 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     9721 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     9781 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     9841 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     9901 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     9961 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
    10021 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
    10081 ctcctcttcc tcatctccgg gcctttcgac ctgcagcagc acgtgttgac aattaatcat
    10141 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atgggatcgg
    10201 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
    10261 gctatgactg ggcacaacag acgatcggct gctctgatgc cgccgtgttc cggctgtcag
    10321 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
    10381 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    10441 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    10501 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    10561 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    10621 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    10681 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
    10741 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
    10801 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
    10861 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
    10921 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
    10981 acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct attaaacaat
    11041 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
    11101 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
    11161 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
    11221 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
    11281 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
    11341 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
    11401 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
    11461 cagaaagctt acctaggcct gaatcgaagt tcctattctt caaaaggtat aggaacttcg
    11521 gttacaagac aggtttaagg agaccaatag aaactgggca tgtggagaca gagaagactc
    11581 ttgggtttct gataggcact gactctctct gcctattggt ctattttccc acccttaggc
    11641 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
    11701 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
    11761 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
    11821 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
    11881 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
    11941 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
    12001 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
    12061 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
    12121 ccccagcact cgtccgaggg caaaggaata gtgatcataa tcagccatac cacatttgta
    12181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    12241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    12301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    12361 aaactcatca atgtatctta ccccaactgg ggtaaccttt gagttctctc agttggggga
    12421 aaggcgcggc cgcgatcttt tgtctctctc tcctcaaata aactgatgcc ttaaatggct
    12481 gtctgagcat cccagtctaa gggtgtccca gctaaggtga ctccttcctg agatttctgc
    12541 catcacccaa gcatctctcc tgagcactac catagagaaa tgtgctgcct tccacctcca
    12601 tcatctttga gttcctcctt cttcagtgaa atgtgccctt agcacttaaa ccctggtttt
    12661 tctcaatgga gagcaaggtg ccacatgtca acttataaga ctgctgaagt gcatcgtatt
    12721 cttcacggat cattctattt tcccgcaaac taaaaataag atttttgcag aaaatactac
    12781 gctcctaact acccagtagt aagttgtaaa tgaaatacta agcagttaag gtttctctga
    12841 gccctgttct catgtatgta aacaatatag ctacaataat ctcttgcctt atattttaca
    12901 gggtagatct aaaggtatta tatccattaa aatgccttat aaagacatgt gccactaggt
    12961 ggacacaggg acaggaaaca atcacaccca ccgaggaggt taaaaacaga tcatcctagc
    13021 catttctaat cagaaaacct atcatcattt atattggtgt acgatgttct cacatgattt
    13081 cacttagata tagataaatt agtttcatat ctaggatgtt ccatgccctt atcagatcta
    13141 ccagaacagc tcctagattt ccatgttcct aactggtggt aattgtatct aaaatgtgct
    13201 acattttcac cagtaaagta tagtcagtct ctgacttcca ctcaaatgtc agtgaagtca
    13261 agtgttgctc tgaaaactag gagcaattga ttatgatact gtgagaaaca aatgctgtta
    13321 tcccatacag ttgtttccaa attgcctagc atcagtactc ctgctgatac ttttaatgat
    13381 actggaaagc aggcaacatg atctgaggaa tacagttctt agtgattgct atataacagg
    13441 ttgcccaagc cgcaatatct taaaatccta aacaacttat ttagctctca gttctttggc
    13501 atttaagtta ggtctggcag aaagttttaa tcttggctag tctgtaattg tggtcaactg
    13561 tcgctccact gggaaccagt gggccatctc ttgctgagcc ttcctctggc tccatatgac
    13621 ctttccttct gcagcaagcc atttagagat tgttaatatc ctcttcaaag caaggctact
    13681 cgctttggcc acatcatatt ggccaatgta actctcacac caatccacat ccaaggatga
    13741 ggaaacagat tcaacctctt gggaaaactt ccagatcact taaccaaggt tatagatgct
    13801 gtgctgctga acggaaaggc tgccttcaca attactctga aattctcaga gatataaaga
    13861 gttctttctc tcctctgggt cctcggagtc taataaaagg aaaggttaag acagagttgt
    13921 ctctgtctta gaaaagaccc tggatggata ttctccacag gagcacaaga aggattggaa
    13981 tatcagaagt aatagagctg gcgcgtgcta acagaatgtg gtagcctgtt gatgaaactg
    14041 accttaatcc tgaaatgtca aaactaatcc tactcctcca aagcaagagg attgtagcca
    14101 ctacccttaa accctaaata cccagagctt ggaagaaagc attttaacat acaaaatggg
    14161 attggctcct cagctagcac ttgataagta cctcttgtct gaaacttctg tgccttcctt
    14221 cccatctttt atttgttgct attttgtgaa gatttacact agattttatt ttcttgtggg
    14281 agtctttatt ttgcttttct taccaagctt ttctgttcaa aaatatactg ttctctttgc
    14341 taacggggca aacgagactg attagtcaag gatgaagatg aaattatgtg tgtcagagac
    14401 agcttaagta tatacaagat gtttcctggg tgtctggagg cgtggggtgt aggaggtgac
    14461 tgctgtcttc catggcacca gcttgcttct taaaggaacc gtgacaattg ccagtttaaa
    14521 aaggatgaag ggagtgccaa tggcatgtgg ttttaagttg tcagaggctg cataaataga
    14581 aaactctaga gacctaaatc tatgcactca tttgttatta aaggcaaaac tccaaactag
    14641 gccaaataca tcctttaata agtttcagat tttatacccc cgaccaaaaa tctccttgtt
    14701 ttccatgatt gtagcatcct tgaatttcta ttctctgaat ttatacacct tcagtgcagg
    14761 tagtctggct cctcaacctt cagactaacg agtggttctt gggtggctat tcctcaacca
    14821 actgtcctag cctcatttta ttccagaaca ataactgcaa accaaaaaga aactttggag
    14881 tgccttggaa atgcttttcc ttaggtagag gatagtgcag tctggtccct cggaatataa
    14941 aacttcaata aagaagtgca tctgggaatg tgagttcaga gacaggagag agggatggaa
    15001 aaatgtaaag actccttggg aagtgatgcc aaggaggtat gatgaagtga gccaagttat
    15061 taggtacctg ggcatttggg tctgagagat agcagagaag caggtggaat gtgaatcaag
    15121 gctattttcc tgggcaaaga catggagagg cactttcttt ggccatgtat tgtcatgggg
    15181 tagcattgag accttctacg tgacttgtgc acttgaaaac ccaaaacagt actataccaa
    15241 caggaccaac atgacaggtg aagctgaggt gcacagagcc aattcatcct tctgtaagcg
    15301 tgttaaggtc tgtttgaaac ttattgccag cactatgatt ggtatggcat cagatcaaaa
    15361 gaatttgaag gtacatataa ggagcattgc tgtgagaaaa acatgaagga aaatcaagcc
    15421 agcaaggtcc ccatcacagc ctaacatcca gctattaaga ttgctttctg gctcaaccct
    15481 agatggacca ttcatatccc aaaagttcct caattgcctg ctccacctac agagttttaa
    15541 ctgagatttt atttgaaaga gctctgctgg taaaacaaaa aatacaaaaa gcagggaaga
    15601 agaaggaaag aaacattttg ttattatttt ggaagtattt gtttgtttta agacaggatc
    15661 ttcatctgtt accaaggcta catagaaact cactatatca gccaggctgg tctcgtattc
    15721 atagtaataa ccctatctcc tgccacgact agaatgatat ccacttctgt aggacaattt
    15781 ggaggacaga tgttgttgtt ggattaaaga tatggatgag cacaaaaaag taaataatta
    15841 aaaataaaaa tgttttttaa aaggcagaaa aatgatttaa ttgtgtttgc ctagattatg
    15901 aaatccaaaa gctggagaat gtacgtgaag taaaagacaa ggaagggata ggcagaggct
    15961 agggtaaaat gatcattcta gaagatagca caggtgactc gacacagtga tagtctatgg
    16021 atgaaaacgc atcgtcaaag caagcatagt caagaagtca acagtagtga tagacacatt
    16081 gcctgcccca ggcaggtagg acagaaagat gcgtataaag cacacaaaca gggcttttat
    16141 ttgcatagca ggaaagcaca ttctaatcta aggattactt tcctgggatc agcttatatt
    16201 cacagttcat aaagaaagat ggcaggcacc atttacaagg ttagaagagt tggactgctt
    16261 gacttgagtc ataggttttg cctgaaaaag aaaagggaag aggaaatgat acttcgaagg
    16321 tgtacagcat acaatcgatt ggactcgcga aggttctcga gacgcgtctc gacgtttaaa
    16381 cttaattaac accgcggtgg agctccagct tttgtcccct ttagtgaggg ttaattgcgc
    16441 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    16501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    16561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    16621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    16681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    16741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    16801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    16861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    16921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    16981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    17041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    17101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    17161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    17221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    17281 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    17341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    17401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    17461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    17521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    17581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    17641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    17701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    17761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    17821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    17881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    17941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    18001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    18061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    18121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    18181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    18241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    18301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    18361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    18421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    18481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    18541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    18601 tgccac
//
