LOCUS       sequence-100489-         941 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-Bla
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      458..467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             464..862
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAMPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 aacgagatga acagcccctc aaaaatatgg ccgccgaggc tggacggccg tgccccagca
       61 gcaccgcctc cgcgccccac gtgatctctc gccgggcaca gcgctgaccg cggaggtcca
      121 accggaagaa tgtccggatt ggacattcgg aagagggccc gccttccctg gggaatctct
      181 gcgcacgcgc agaacgcttc gaccaatgaa aacacaggaa gccgtccgcg caaccgcgtt
      241 gcgtcacttc tgccgcccct gtttcaaggt atatagccgt agacggaact tcgcctttct
      301 ctcggcctta gcgccatttt tttgggtgag tgttttttgg ttcctgcgtt gggattccgt
      361 gtacaatcca tagacatctg acctcggcac ttagcatcat cacagcaaac taactgtagc
      421 ctttctctct ttccctgtag aaacctctgc acctgaggcc accatggcta tgcctttgtc
      481 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
      541 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
      601 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
      661 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
      721 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
      781 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
      841 tggttatgtg tgggagggct aattcgaagg cctgtcgtga attcactcct caggtgcagg
      901 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct g
//
