LOCUS       sequence-100648-        1003 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GH
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        352..664
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    360..378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    396..414
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    431..449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    467..485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    503..521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    538..556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    574..592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     637..661
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      737..746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattgga
       61 tccctataca gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt
      121 ttttcaacta ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga
      181 catctacttt gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt
      241 cacttataat tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttcg
      301 actcctctgc agaatgcggc gatgtttcgg taaggggtcc gctatctaga cgagtttact
      361 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      421 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      481 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      541 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      601 cgattaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga accgtcagat
      661 cgcctggagc caattccaac tcttttgcct tatacctact ttccgtacca cttcctaccc
      721 tcgaaaggcc tctgaggcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc
      781 attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta
      841 cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta
      901 cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa
      961 tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cag
//
