LOCUS       sequence-100697-         902 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RH
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..618,619..624,625..660,661..663,664..699,700..702,
                     703..738,739..744)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             751..807
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
       61 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
      121 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
      181 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
      241 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
      301 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
      361 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
      421 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
      481 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
      541 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
      601 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
      661 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
      721 tttgaccttg acatgctccc cgggtccggc gctactaact tcagcctgct gaagcaggct
      781 ggtgacgtcg aggagaatcc tggtcccatg aaaaagcctg aactcaccgc gacgtctgtc
      841 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
      901 ga
//
