LOCUS       sequence-100943-         883 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtggcggccg ctcgagtagc catggcttcc cgccggcggt ggcggcgcag gatgatggca
       61 cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctt
      121 ctgctaggat caatgtgtag taatgagttt aaggcgcgcg gctctagagc tgaagcccga
      181 tattcaggat cttcttcaag actttctact tagaacctgc cgtgttatcg agcttaccta
      241 ttgttttttt ccaaatgatt atttttgtta gtttacatac agtttagtgc ctagaaaatg
      301 tgcctttgtt tttcatatac actccaaact ggaggtgtga cctaccctcc ccacattttg
      361 cacagtgcct ttacagattt ctggactggg aaagaggact tgtgtagttc tgagtgctga
      421 atggaggtgg atgttgtcac tcaagatgac ttgttgtgga ttccaggaag ggggttttgt
      481 tcaaggcagc tgtccccaca tctgacatca tcttcaaaac cacattttaa acttttgggt
      541 aattagattc ccaacttgga ccttccaaga agaacactac tgcctaacag caatgggtga
      601 caatagcagg gtgtgcctta ttttgatagc taggtaactg ttctttaaga ttattctccg
      661 tttgtaagca ctactgactc ccgctgtatt taagatgctg gtgaagagct catgctctta
      721 tgttagatat gactatgttt actttcttgt tcttgttgta tatcactcct aaaatagtat
      781 tttaaccaga aataaccctt taagaagttt ttatagaact atgaccaaag tttctatttt
      841 gccaaaagtt aagtacaaat aaattgcccc aatagataca ttc
//
