LOCUS       sequence-101502-         840 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Human transferrin receptor tagged with pH sensitive orange/red 
            fluorescent protein pHoran4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: TfR-pHoran4
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(57..767)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKKRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(335..354)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ataacagttg ggccatggcg gccaagcttc tgcaggtcga ctctagatta gctagcttac
       61 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
      121 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgccgac gatgtaggcg
      181 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcgga ggtgtagtgg
      241 ccgccgtcct tcagcttcag cctcttcttg atctcgccct tcagggcgcc gtcctcgggg
      301 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
      361 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
      421 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
      481 aagccctcgg ggaaggacag cttgaagtag tcggggatgt cggcggggtg cttcacgtag
      541 gccttggagc cgtaggtgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
      601 cccttggtca ccttcagctt agcggtctga aagccctcgt aggggcggcc ctcgccctcg
      661 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgcgcacctt gaagcgcatg
      721 aactccttga tgatggccat gttattctcc tcgcccttgc tcaccatgac cggtggatcc
      781 ccaaactcat tgtcaatgtc ccaaacgtca ccagagaggg catttgcagc tccctgaata
//
