LOCUS       sequence-101942-         959 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of the horse sweat/saliva protein Latherin as a
            His-tagged thioredoxin fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Vance SJ, McDonald RE, Cooper A, Smith BO, Kennedy MW
  TITLE     The structure of latherin, a surfactant allergen protein from horse 
            sweat and saliva.
  JOURNAL   J R Soc Interface. 2013 Jun 19;10(85):20130453. doi: 
            10.1098/rsif.2013.0453. Print 2013 Aug 6.
  PUBMED    23782536
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J R Soc 
            Interface."; date: "2013-06-19"; volume: "10(85):20130453. doi"; 
            pages: " 10.1098/rsif.2013.0453. Print 2013 Aug 6"
COMMENT     Sequence Label: pET32-sLatherin
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(734..748)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(764..808)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(815..832)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(842..859)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccttatta aacgctcaga tccacgttcg
      121 cacgatccag gatcaggtta atcaggttgt tcaccagctg cagatccagg ttgctcacca
      181 ggctgttcag ggtcgggcac agacccgcac ccagatcttc ggtaataaag ttgcccagcg
      241 cgttttcaat ggtgcccagc accgcattca ccggcagaga cagcggattg ccgctctgca
      301 gctcaatcgc acgcggcagc aggctgcaat ggccaaaggc cagacgatat ttgccatctt
      361 catcagattc cagacgcagc tgcacacgaa tatcggtacg cacatacagg gtcagcggtt
      421 ccagaatcag cagtttcaga tacacagaca gttccagcgg aatccaaata tcaatgcctt
      481 tgctatccgg gctaaattcc agagacagtt gcagcagacg ggtatcctgc agtttaattt
      541 ccgcatcaga cggacccagc agcgggccca ccagaatatt cagcaggccg gtcacctgat
      601 ccagaatggt gttcagcaga ccttccaggt taatcgcgtt cagcaggctc agaaagttgc
      661 catccagcag gccctgggtc agcgcatcgg taatctggct gctcacttcc ggcggaattt
      721 gttgagccat ggccttgtcg tcgtcgtcgg tacccagatc tgggctgtcc atgtgctggc
      781 gttcgaattt agcagcagcg gtttctttca taccagaacc gcgtggcacc agaccagaag
      841 aatgatgatg atgatggtgc atatggccag aaccagaacc ggccaggtta gcgtcgagga
      901 actctttcaa ctgaccttta gacagtgcac ccactttggt tgccgccact tcaccgttt
//
