LOCUS       sequence-101947-         948 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Jullien N, Sampieri F, Enjalbert A, Herman JP
  TITLE     Regulation of Cre recombinase by ligand-induced complementation of 
            inactive fragments.
  JOURNAL   Nucleic Acids Res. 2003 Nov 1;31(21):e131.
  PUBMED    14576331
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2003-11-1"; volume: "31(21)"; pages: "e131"
COMMENT     Sequence Label: pcDNA_DiCre_60.F2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..179
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             186..461
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagga tcgatccact agtccagtgt ggtggaattc
      121 tgcagatatc cagcacagtg gcggccgcca ccatggcccc taagaagaag agaaaggtgt
      181 ctagaatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact
      241 ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg
      301 aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa
      361 tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc
      421 aagcctggga cctctattat catgtgttcc gacgaatctc agctagcccc agcaaccccg
      481 gcgccagcaa cggatccaac aggaaatggt tccctgctga acctgaggat gtgagggact
      541 acctcctgta cctgcaagcc agaggcctgg ctgtgaagac catccaacag cacctgggcc
      601 agctcaacat gctgcacagg agatctggcc tgcctcgccc ttctgactcc aatgctgtgt
      661 ccctggtgat gaggagaatc agaaaggaga atgtggatgc tggggagaga gccaagcagg
      721 ccctggcctt tgaacgcact gactttgacc aagtcagatc cctgatggag aactctgaca
      781 gatgccagga catcaggaac ctggccttcc tgggcattgc ctacaacacc ctgctgcgca
      841 ttgccgaaat tgccagaatc agagtgaagg acatctcccg caccgatggt gggagaatgc
      901 tgatccacat tggcaggacc aagaccctgg tgtccacagc tggtgtgg
//
