LOCUS       sequence-102157-         766 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of artificial 
            microRNAs into rice MIR390 precursor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pENTR-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(173..272)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(289..308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(290..308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(313..329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(313..329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(431..450)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(509..528)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg cacctcgacg gtctcacatg
      121 gtttgttctt accacacgac caattaaatc gagctcaagg gtgggcgcgc cgacccagct
      181 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
      241 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
      301 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
      361 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      421 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
      481 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      541 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
      601 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
      661 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      721 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattag
//
