LOCUS       sequence-102440-        9351 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Inducible expression of Ngn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams 
            D, Sanna PP, Baldwin KK
  TITLE     Selective conversion of fibroblasts into peripheral sensory neurons.
  JOURNAL   Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 
            Nov 24.
  PUBMED    25420069
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3887"; 
            journalName: "Nat Neurosci"; date: "2015-01"; volume: "18"; issue: 
            "1"; pages: "25-35"
COMMENT     Sequence Label: Teto Ngn2
FEATURES             Location/Qualifiers
     source          1..9351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2599..2617,2618..2640,2641..2659,2660..2682,2683..2701,
                     2702..2724,2725..2743,2744..2766,2767..2785,2786..2808,
                     2809..2827,2828..2850,2851..2869)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2902..2940
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2937..2961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4041..4629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4094..4114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4512..4523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4632..4648)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4633..4649)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5154..5334
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5360..5377)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5366..5590
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5636..6064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5723..5742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5933..5954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6073..6093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6078..6407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6258..6393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6320..6339
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6455..6502
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6521..6895
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7025..7146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7062..7081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7116..7135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7195..7211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7208..7230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7219..7235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7243..7249,7250..7267,7268..7273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7288..7309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7426..7443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7597..8185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7677..7696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8356..9147,9148..9216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8979..8998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9217..9321)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaaaggcg tatcacgagg ccctttcgtc
     2581 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2761 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2821 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2881 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
     2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3001 agacaccggg accgatccag cctcctgcag gtctagagga tcgaattcgc caccatgttc
     3061 gtcaaatctg agactctgga gttgaaggag gaagaggagg tactgatgct gctgggctcg
     3121 gcttccccgg cctcggcgac cctgaccccg atgtcctcca gcgcggacga ggaggaggac
     3181 gaggagctgc gccggccggg ctccgcgcgt gggcagcgtg gagcggaagc cgggcagggg
     3241 gtgcagggca gtccggcgtc gggtgccggg ggttgccggc cagggcggct gctgggcctg
     3301 atgcacgagt gcaagcgtcg cccgtcgcgc tcacgggccg tctcccgagg tgccaagacg
     3361 gcggagacgg tgcagcgcat caagaagacc cgcaggctca aggccaacaa ccgcgagcgc
     3421 aaccgcatgc acaacctaaa cgccgcgctg gacgcgctgc gcgaggtgct gcccaccttc
     3481 cccgaggatg ccaagctcac gaagatcgag acgctgcgct tcgcccacaa ttacatctgg
     3541 gcgctcaccg agactctgcg cctggcggac cactgcgccg gcgccggtgg cctccagggg
     3601 gcgctcttca cggaggcggt gctcctgagc ccgggagctg cgctcggcgc cagcggggac
     3661 agcccttctc caccttcctc ctggagctgc accaacagcc cggcgtcatc ctccaactcc
     3721 acgtccccat acagctgcac tttatcgccc gctagccccg ggtcagacgt ggactactgg
     3781 cagcccccac ctccggagaa gcatcgttat gcgcctcacc tgcccctcgc cagggactgt
     3841 atctagagct gcgggtctcc ctctctcgtc ctctacccgg ccctcttccc atccttctcc
     3901 cgcccctcac cctccacgcc ccggactcca cttcacagag cagaggtggc ccttgcaatc
     3961 ccctcggcgg ctggtgcatt cgggggtgga gaccagctct ggtttggatc cgttaacaat
     4021 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4441 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4561 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4621 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     4681 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     4741 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     4801 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4861 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     4921 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     4981 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5041 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5101 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5161 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5221 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5281 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5341 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5401 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5461 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5521 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5581 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6061 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6421 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6481 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6541 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6601 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6661 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     6721 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     6781 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     6841 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     6901 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6961 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7021 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7081 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7141 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaca c
//
