LOCUS       sequence-102517-         809 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQN
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggttccgcg tggtagtgac ctccaggagg atgaggatgg tgtttatttc agctcatacg
      121 ggcattatgg gatacatgaa gaaatgctaa aggacaaaat acgaacagaa agctaccgag
      181 atttcatata ccaaaatcca catatcttca aagacaaggt agttttggat gttgggtgtg
      241 gaactggaat tctctctatg tttgctgcta aagctggggc gaagaaggtt cttggagttg
      301 atcaatctga aatactttac caggcaatgg atattataag actaaataaa cttgaagata
      361 ctattacact aattaaagga aagattgaag aagttcatct tcctgtagaa aaagtagatg
      421 ttatcatatc tgagtggatg ggctattttc ttctgtttga gtctatgtta gattctgtcc
      481 tttatgcaaa gaacaaatac ttggcaaaag gaggctcggt ctaccctgac atttgcacta
      541 tcagccttgt agcagtgagt gatgtgaata aacatgctga tagaattgct ttttgggatg
      601 atgtctatgg cttcaagatg tcctgcatga agaaagcagt tattccagaa gctgttgtgg
      661 aagttttaga tccgaagact cttatttcag aaccttgtgg tattaagcat atagattgcc
      721 atacgacgtc tatctcagat ttggaatttt catcagattt taccctgaaa atcacaagga
      781 catccatgtg cacggcaatt gctggctac
//
