LOCUS       sequence-102602-         722 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express E2 UbcH5B in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, 
            Wolberger C
  TITLE     E2 ubiquitin-conjugating enzymes regulate the deubiquitinating 
            activity of OTUB1.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. 
            Epub 2013 Aug 18.
  PUBMED    23955022
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2655";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1033-9"
COMMENT     Sequence Label: UbcH5B
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatggg ccatcatcat catcatcatc cgtcaggagt caagactgag
      181 aacaacgatc atattaattt gaaggtggcg gggcaggatg gttctgtggt gcagtttaag
      241 attaagaggc atacaccact tagtaaacta atgaaagcct attgtgaacg acagggattg
      301 tcaatgaggc agatcagatt ccgatttgac gggcaaccaa tcaatgaaac agacacacct
      361 gcacagttgg aaatggagga tgaagataca attgatgtgt tccaacagca gacgggagga
      421 ggtatggctc tgaagagaat ccacaaggaa ttgaatgatc tggcacggga ccctccagca
      481 cagtgttcag caggtcctgt tggagatgat atgttccatt ggcaagctac aataatgggg
      541 ccaaatgaca gtccctatca gggtggagta tttttcttga caattcattt cccaacagat
      601 taccccttca gaccacctaa ggttgcattt acaacaagaa tttatcatcc aaatattaac
      661 agtaatggca gcatttgtct tgatattcta cgatcacagt ggtctccagc actaactatt
      721 tc
//
