LOCUS       sequence-102675-         719 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  express a human E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, 
            Wolberger C
  TITLE     E2 ubiquitin-conjugating enzymes regulate the deubiquitinating 
            activity of OTUB1.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. 
            Epub 2013 Aug 18.
  PUBMED    23955022
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2655";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1033-9"
COMMENT     Sequence Label: UbcH5A
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..68)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgatt aacccttttc gaactgcggg
       61 tggctccagg cctcgattac attgcatatt tctgagtcca ttctcttgca tgtctgttgt
      121 atttttcttt gtctgattta tagatttgtg caatatctgg tactaagggg tcatctggat
      181 taggatcaca aagtagagaa catatggaca ataaaacttt tgatacagtc agagctggtg
      241 accattgtga cctcagaata tcgagacaaa tacttccatt actgtttatg tttggatggt
      301 aaatttttgt tgtgaaagca atctttggtg gtttaaaagg ataatctgtc ggaaaatgta
      361 cagtgagaaa gaagactcca ccttgatatg cgctatcagg aggccccata atagtggctt
      421 gccagtggaa caagtcatct cccacaggtc cagctgaaca gtgagcaggt ggatcgcgct
      481 gtagatcact caattctttc tgaatcctct tcagcgccat acctcctccc gtctgctgtt
      541 ggaacacatc aattgtatct tcatcctcca tttccaactg tgcaggtgtg tctgtttcat
      601 tgattggttg cccgtcaaat cggaatctga tctgcctcat tgacaatccc tgtcgttcac
      661 aataggcttt cattagttta ctaagtggtg tatgcctctt aatcttaaac tgcaccaca
//
