LOCUS       sequence-102746-         878 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian Expression of Tiam DH/PH-tgRFPt-Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(80..199,200..202,203..409))
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt ggaaccggaa
       61 cccttaaaca tgtttaacca atattcagct cgtcatagat ttcttctggt tcgaacatca
      121 caccatcgcc gttctcgcga gcgtaaatgg ccagagcggc acccatcggg atatacagtt
      181 cacgagacac gcccttaaac tgggcgttga actggataaa atcatttgtc agttgcaggt
      241 tgccggtcgc acttgcagac agattcagca cgatctgacc gtctttcaca tactccacgg
      301 gcacgttcac gcccaggtat gtggcatcca ccaccagata tggggtaaag ctgttatcaa
      361 ccagccaatc gtaatattca cgcagcagct tagggcgttt cggggagctg aattcctgca
      421 gggtcaggcc gctcagatca atgccgaatt cgttcgaagc ttgagctcga gatctgagtc
      481 cggacttgta cagctcgtcc atgccattaa gtttgtgccc cagtttgcta gggaggtcgc
      541 agtatctggc cacagccacc tcgtgctgct cgacgtaggt ctctttgtcg gcctccttga
      601 ttctttccag tctgtggtcc acatagtaga cgccgggcat cttgaggttc ttagcgggtt
      661 tcttggatct gtatgtggtc ttgaagttgc agatcaggtg gcccccgccc acgagcttca
      721 gggccatgtc ggttctgcct tccaggccgc cgtcagcggg gtacagcatc tcggtgttgg
      781 cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgggaag ttcacccctc
      841 tgatcttgac gttgtagatg aggcagccgt cctggagg
//
