LOCUS       sequence-102754-         548 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Template for generating gene 60 mRNA truncated two codons after 
            coding gap + Cys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 thru gap + Cys
FEATURES             Location/Qualifiers
     source          1..548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(335..351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(335..351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(348..370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    359..375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(383..389,390..407,408..413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    428..449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc tgatgaaaag
       61 ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg atatgaaaaa
      121 atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga actatgcgaa
      181 tgtcgctatt atgacagacg cagatcacga tggatagcct tcgggctatc tatagaaata
      241 cctcataatt aagagattat tggattaggt tgttagttta ctttaccaca aggatgtggt
      301 ataattaatt gggcaaaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      541 tgcgtatt
//
