LOCUS       sequence-102943-         934 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expression of cdc42 constitutively active mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB cdc42 QL
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(117..296,297..299,300..692)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(745..763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(783..800)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    789..900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc gccaccatgc
      121 agacaattaa gtgtgttgtt gtgggcgatg gtgctgttgg taaaacatgt ctcctgatat
      181 cctacacaac aaacaaattt ccatcggaat atgtaccgac tgtttttgac aactatgcag
      241 tcacagttat gattggtgga gaaccatata ctcttggact ttttgatact gcagggctcg
      301 aggattatga cagattacga ccgctgagtt atccacaaac agatgtattt ctagtctgtt
      361 tttcagtggt ctctccatct tcatttgaaa acgtgaaaga aaagtgggtg cctgagataa
      421 ctcaccactg tccaaagact cctttcttgc ttgttgggac tcaaattgat ctcagagatg
      481 acccctctac tattgagaaa cttgccaaga acaaacagaa gcctatcact ccagagactg
      541 ctgaaaagct ggcccgtgac ctgaaggctg tcaagtatgt ggagtgttct gcacttacac
      601 agaaaggcct aaagaatgta tttgacgaag caatattggc tgccctggag cctccagaac
      661 cgaagaagag ccgcaggtgt gtgctgctat gaattcccgg gtcgactcga gcggccgctc
      721 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      781 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      841 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      901 gcattgtctg agtaggtgtc attctattct gggg
//
