LOCUS       sequence-102971-         699 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  2x-30nm SAH scaffold with EF Hand and split GFP attachment sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, 
            Sivaramakrishnan S, Verhey KJ
  TITLE     A method for multiprotein assembly in cells reveals independent 
            action of kinesins in complex.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086.
            Epub 2014 Nov 3.
  PUBMED    25365993
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407086"; journalName: "J Cell Biol"; date: 
            "2014-11-10- 10"; volume: "207"; issue: "3"; pages: "393-406"
COMMENT     Sequence Label: SNAP-EF(N)-60nmSAH-GFP(1-10)
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggacaagga ttgtgaaatg
       61 aaacgcacca cactggacag ccctttgggg aagctggagc tgtctggttg tgagcagggt
      121 ctgcacgaaa taaagctcct gggcaagggg acgtctgcag ctgatgccgt ggaggtccca
      181 gcccccgctg cggttctcgg aggtccggag cccctgatgc aatgcacagc ctggctgaat
      241 gcctatttcc accagcccga ggctatcgaa gagttccccg tgccggctct tcaccatccc
      301 gttttccagc aagagtcgtt caccagacag gtgttatgga agctgctgaa ggttgtgaaa
      361 ttcggagaag tgatttctta ccagcaatta gcagccctgg caggcaaccc cgcagccacg
      421 gcagcagtgg gaggagcaat gagaggcaat cctgtcccta tcctgatccc gtgccacaga
      481 gtggtctgca gcagcggagc cgtgggcggt tacgagggtg gactggccgt gaaggaatgg
      541 cttctggccc atgaaggcca tcggttgggg aagccaggct tgggagggag ctcaggtctg
      601 gcaggggcct ggctcaaggg agcgggagct acctcgggct ccccgcctgc tggccgaaac
      661 gtgtacaagt ccggaagcgg tggatcaggc gctagcatg
//
