LOCUS       sequence-103027-         544 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pre-2Ato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..150
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 aatggatcca tgccaccttc tactagtctt ttgttgcttg ctgcacttct ccccttcgct
       61 cttcccgctt cagattggaa gacaggagaa gtcaccgctt cccgcgacca catggtgctg
      121 cacgaatatg tgaacgctgc tggcatcaca ggcggtggcg gatctggcgg cggtggtagc
      181 gtcgactttc agaaagctag ctctattgtt tataagaagg agggggaaca ggtggagttc
      241 agtttcccat tggccttcac agtcgagaag ctcacaggat caggagagtt gtggtggcag
      301 gccgaaagag cctcctccag caagagttgg atcacttttg atctgaaaaa caaagaagtg
      361 agcgtgaaga gagtcacaca ggaccctaag ctgcagatgg gcaagaagtt gcctttgcac
      421 ttgacactcc cccaagccct gccccagtat gctggctccg ggaacctcac cctcgccttg
      481 gaggctaaga ccggaaagtt gcatcaggag gtcaacctcg tggtgatgag ggcaacacag
      541 ctgc
//
