LOCUS       sequence-103041-         536 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentivirus expressing long isoform of human DIXDC1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
  TITLE     An AMPK-independent signaling pathway downstream of the LKB1 tumor 
            suppressor controls Snail1 and metastatic potential.
  JOURNAL   Mol Cell. 2014 Aug 7;55(3):436-50. doi: 
            10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
  PUBMED    25042806
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.021"; journalName: "Mol Cell"; date: 
            "2014-08-7- 7"; volume: "55"; issue: "3"; pages: "436-50"
COMMENT     Sequence Label: pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccaccat ggattacaag
      121 gacgatgacg acaaactcgg atccatggga accaattcag tagacatgct agcctgcctg
      181 acccgaggga acttactgga cgtcctgcag gagggcttca atgagcaaca gctgcaggcc
      241 tatgtggcct gggtgaatgc acagctgaag aagaggccag cagtgaagcc tgtgcaggac
      301 ctgcgacaag atctccggga tggggtgatc ctggcatatc tcatcgagat tgttgcagga
      361 gaaaagctga gtggggtaca gctgagtccc ggtaaccaac aggagatgaa gaataatgtg
      421 gagaaagtgc tacagtttgt ggcctctaaa aagattcgta tgcaccaaac ttcggctaaa
      481 gatattgtgg atggaaacct gaagtctatc atgaggctgg tccttgcctt ggcagc
//
