LOCUS       sequence-103079-         764 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  E. coli expression of SspB Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pQE-80L MBP-SspB Micro
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..164,165..167,168..374))
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDXLNI"
     CDS             complement(378..398)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(465..488)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 atctatcaac aggagtccaa gctcagctaa ttaagctttt aaccaatatt cagntcgtca
       61 tagatttctt ctggttcgaa catcacacca tcgccgttct cgcgagcgta aatggccaga
      121 gcggcaccca tcgggatata cagttcacga gacacgccct taaactgggc gttgaactgg
      181 ataaaatcat ttgtcagttg caggttgccg gtcgcacttg cagacagatt cagcacgatc
      241 tgaccgtctt tcacatactc cacgggcacg ttcacgccca ggtatgtggc atccaccacc
      301 agatatgggg taaagctgtt atcaaccagc caatcgtaat attcacgcag cagcttaggg
      361 cgtttcgggg agctggatcc ctgaaaatac aggttttcgg tcgttccctc gatcccgagg
      421 ttgttgttat tgttattgtt gttgttgttc gagctcgaat tagtctgcgc gtctttcagg
      481 gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac
      541 cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccata
      601 gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca
      661 cccagcggtt tgtctttatt aaccgcttcc agaccttcat cagtcagcag atagttttcg
      721 aggaactctt ttgccagctc tttgttcgga ctggcggcgt taat
//
