LOCUS       sequence-103291-        1294 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Nuclear - Nucleolus, N1 Cloning Vector, 
            Excitation:580, Emission:608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FusionRed-FTR940-N-11
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        254..353
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     304..324
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      539..548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             545..1243
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtatacaaca tatggccaca ggtcgccctc ttattgagcg gtcaatgacg gtaaatggcc
       61 cgctggcata ggccagatag cactgacctg tgggaacttt ctactggcag ataccattct
      121 acgtatgtag gtcatcgcta ttaaccatgg tgatggcggt ttggcagata catcaatggg
      181 cggggatagc ggtttgactc cacgggattt ccaagtctcc acccccattg acgtcatggg
      241 agtttgtttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      301 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      361 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttatgggcat
      421 ggcgcgacca cggggcgcgg ggccctgcag ccccgggttg gagcgggctc cgcgccggag
      481 cgtcggggag ctgcgcctgc tcttcgaagc gggcggatct ggatcggatc caccggtcgc
      541 caccatggtg agcgagctga ttaaggagaa catgcccatg aagctgtaca tggagggcac
      601 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
      661 ccagaccatg agaatcaagg tcgtcgaggg cggccctctc cccttcgcct tcgacatcct
      721 ggctaccagc ttcatgtacg gcagcagaac cttcatcaag caccctccgg gcatccccga
      781 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
      841 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
      901 cgtcaaggtt agaggggtga acttcccagc caacggccct gtgatgcaga agaaaacact
      961 cggctgggag gcctccaccg agacgatgta ccccgctgac ggcggcctgg aaggcgcatg
     1021 tgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaaccttg agaccacata
     1081 cagatccaag aaacccgcta cgaacctcaa gatgcccggc gtctacaacg tggaccacag
     1141 actggaaaga atcaaggagg ccgacgatga gacctacgtc gagcagcacg aggtggctgt
     1201 ggccagatac tctactggtg gcgctggtga tggaggtaaa tgagcggccg cgactctaga
     1261 tcataatcag ccataccaca ttgtagaagt tccc
//
