LOCUS       sequence-103346-        1184 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Centrosomes, C1 Cloning Vector, Excitation:487, 
            Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TPX2-M2-C-18
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(44..46,47..49,50..760)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(89..110)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(350..369)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     697..718
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagtagtg acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga
       61 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      121 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      181 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac
      241 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      301 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      361 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      421 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      481 caacagccac aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt
      541 caagacccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      601 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      661 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      721 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
      781 tcaagcttcg ggagatagtg gtgggagcgg ttcatcacaa gttaaaagct cttattccta
      841 tgatgccccc tcggatttca tcaatttttc atccttggat gatgaaggag atactcaaaa
      901 catagattca tggtttgagg agaagccaat ttggagaata agttactggg gagatgtact
      961 ggagggcttt ttcaggcaaa ctctttgaga agctatctca gcagctattg tcacaccttt
     1021 gaaacagtga caacacttac tacagagcag aaaagaaaat ctggtggaca tccatcggtc
     1081 aatgcttgtt ctttcctgaa gtgaagcagc caatcagaaa actccagcag ctcgagagaa
     1141 tttcctagct tctgctcgaa ggatggacgc acggaagcct cagt
//
