LOCUS       sequence-103462-        1062 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  For mRNA synthesis or expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Matoba S, Liu Y, Lu F, Iwabuchi KA, Shen L, Inoue A, Zhang Y
  TITLE     Embryonic Development following Somatic Cell Nuclear Transfer 
            Impeded by Persisting Histone Methylation.
  JOURNAL   Cell. 2014 Nov 6;159(4):884-95. doi: 10.1016/j.cell.2014.09.055. 
            Epub 2014 Oct 30.
  PUBMED    25417163
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.055"; journalName: "Cell"; date: "2014-11-6-
            6"; volume: "159"; issue: "4"; pages: "884-95"
COMMENT     Sequence Label: pcDNA-flag-mZscan4d-polyA
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttatgga ctacaaagac
      121 gatgacgaca agggatccac tagtaacggc cgccagtgtg ctggaattca cgcttcacag
      181 caggcaccag caaaagacct tcagaccaac aatttagagt ttactccatc tcatagttct
      241 ggtgtgcagt gggtagaaga catctctaac tcaccaagtg ctcagctaaa cttttctcca
      301 agtaacaatg gctgctgggc aactcaggag ctgcaaagtc tctggaagat gttcaactcc
      361 tggttgcagc cagaaaagca gactaaggag cagatgattt ctcaactggt cttggagcag
      421 tttctcctca ttgggcactg caaggacaag tatgctttga cagagaagtg gaaagccagt
      481 ggtagcgata tgaggagatt catggagagt ctgactgatg agtgcttgaa gcctcctgtc
      541 atggtccatg tttcaatgca aggacaagaa gctctctttt ctgaaaacat gccattaaaa
      601 gaagtcatca agcttttgaa acaacagcaa tctgcaacaa ggccaacacc agataatgag
      661 cagatgccag tagacaccac acaagataga ttattggcca caggacaaga aaacagtgaa
      721 aatgaatgca acaactcttg taatgctact gaagcaaatg ttggtgaaag ctgtagtgga
      781 aatgaaatgg actcccttct tattatccag aaagaacagt accctgagca tgaagagggg
      841 aatgttgttt ttcaattccc tcttgatgcc agaagagcaa gtcaaggcaa ctccagtcat
      901 catgtagact tccggagtgc tccgactcct gcggatgtcc ccatggagga acaaccaaag
      961 gatttatcca gagaaaacat ctctgaggac aagaacaatt gctataacac ttccaggaat
     1021 gcagctactc aagtatatag aagtgataat attcccagga aa
//
