LOCUS       sequence-103468-         628 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV L5-L2
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(206..225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(207..225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(270..473)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(299..319)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtacct ctagatgcat gctcgagcgg
      121 ccgccagtgt gatggatatc tgcagaattc cagcacactg gcggccgtta ctagtggatc
      181 cgagctcggt accaagcttg ggtctcccta tagtgagtcg tattaatttc gataagccgg
      241 taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca
      301 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      361 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      421 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      481 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      541 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      601 atatgataca cttgatgtac tgccaagt
//
