LOCUS       sequence-103526-         658 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV tdTomato L5-L2
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     109..125
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     110..126
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(233..256)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(431..450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtacct ctagaggctc gaggtcgacg
      121 gtatcgataa gcttgatatc gaattcttac ttgtacagct cgtccatgcc gtacaggaac
      181 aggtggtggc ggccctcgga gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg
      241 gaggtgatgt ccagcttggt gtccacgtag tagtagccgg gcagttgcac gggcttcttg
      301 gccatgtaga tggtcttgaa ctccaccagg tagcggccgc cgtccttcag cttcagggcc
      361 tggtggatct cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc
      421 cagcccatgg tcttcttctg cattacgggg ccgtcggggg ggaagttggt gccgcgcatc
      481 ttcaccttgt agatcagcgt gccgtcctgc agggaggagt cctgggtcac ggtcaccaga
      541 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      601 ttgtaatcgg ggatgtcggc ggggtgcttc acgtacgcct tggagccgta catgaact
//
