LOCUS       sequence-103558-         696 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV L1-R5
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(250..651)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 tgcagtacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaacttttg tatacaaagt tgtcctctag atgcatgctc
      181 gagcggccgc cagtgtgatg gatatctgca gaattccagc acactggcgg ccgttactag
      241 tggatccggc gactcagtca atcggaggac tggcgcgccg agtgaggggt tgtgagctct
      301 tttatagagc tcgggaagca gaagcgcgcg aacagaagcg agaagcaggc tgattggtta
      361 attcaaataa ggcgcagggt catttcaggt ccttggggga gcctggaaac atctgatggg
      421 tcttaagaaa ctgctgaggg ttgggccata tctggggacc atctgttctt ggcctcgggc
      481 cggggccgaa actgcggtga ccatctgttc ttggccccgg gccggggccg aaactgctca
      541 ccgcagatat cctgtttggc ccaacgttag ctattttcat gtacccgccc ttgatctgaa
      601 cttctctatt cttggtttgg tatttttcca tgccttgcaa aatggcgtta ctgcagctag
      661 cttgccaaac ctacaggtgg ggtctttcag cgaagc
//
