LOCUS       sequence-103606-         697 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6-miR-30 L5-L2
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           complement(118..230)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(244..337)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(392..640)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(450..469)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(620..640)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtacat tagtcttcca attgaaaaaa
      121 gtgatttaat ttataccatt ttaattcagc tttgtaaaaa tgtatcaaag agatagcaag
      181 gtattcagtt ttagtaaaca agataattgc tcctaaagta gccccttgaa ttctggttgc
      241 tcgagccttc tgttgggtta acctgaagaa gtaatcccag caagtgtttc caagatgtgc
      301 aggcaacgat tctgtaaagt actgaagcct cattcaaaca attaccctgt tatccctagt
      361 cgactagtat atgtgctgcc gaagcgagca cggtgtttcg tcctttccac aagatatata
      421 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      481 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      541 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      601 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accctggcgc
      661 gcgctcggcg cgcggtcacg ctccgtcacg tggtgcg
//
