LOCUS       sequence-103661-         873 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expression vector of BRCA1 wildtype siRNA resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             204..227
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttccaccat ggactacaaa gaccatgacg
      181 gtgatacata tgatcatgac atcgattaca aggatgacga tgacaagaca agtttgtaca
      241 aaaaagcagg cttcaccatg gatttatctg ctcttcgcgt tgaagaagta caaaatgtca
      301 ttaatgctat gcagaaaatc ttagagtgtc ccatctgtct ggagttgatc aaggaacctg
      361 tctccacaaa gtgtgaccac atattttgca aattttgcat gctgaaactt ctcaaccaga
      421 agaaagggcc ttcacagtgt cctttatgta agaatgatat aaccaaaagg agcctacaag
      481 aaagtacgag atttagtcaa cttgttgaag agctattgaa aatcatttgt gcttttcagc
      541 ttgacacagg tttggagtat gcaaacagct ataattttgc aaaaaaggaa aataactctc
      601 ctgaacatct aaaagatgaa gtttctatca tccaaagtat gggctacaga aaccgtgcca
      661 aaagacttct acagagtgaa cccgaaaatc cttccttgca ggaaaccagt ctcagtgtcc
      721 aactctctaa ccttggaact gtgagaactc tgaggacaaa gcagcggata caacctcaaa
      781 agacgtctgt ctacattgaa ttgggatctg attcttctga agataccgtt aataaggcaa
      841 cttattgcag tgtgggagat caagaattgt tac
//
