LOCUS       sequence-103756-         760 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  PhoRadAC6 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-172
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             239..261
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(288..572)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(346..365)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gccagcttaa tgatgatgat gatgatggaa ttccgttacg gtgtaggttt tggtagcgtc
       61 gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga aaactttttc
      121 cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt taccgttcag
      181 gataagtttg tactgggtac ctagctgagt attatggaga acaagtccca tatgtatatc
      241 tccttcttaa agttaaacaa aattatttct agcccaaaaa aacgggtatg gagaaacagt
      301 agagagttgc gataaaaagc gtcaggtagg atccgctaat cttatggata aaaatgctat
      361 ggcatagcaa agtgtgacgc cgtgcaaata atcaatgtgg acttttctgc cgtgattata
      421 gacacttttg ttacgcgttt ttgtcatggc tttggtcccg ctttgttaca gaatgctttt
      481 aataagcggg gttaccggtt tggttagcga gaagagccag taaaagacgc agtgacggca
      541 atgtctgatg caatatggac aattggtttc ttctctgaat ggcgggagta tgaaaagtat
      601 ggctgaagcg caaaatgatc ccctgctgcc gggatactcg tttaatgccc atctggtggc
      661 gggtttaacg ccgattgagg ccaacggtta tctcgatttt tttatcgacc gaccgctggg
      721 aatgaaaggt tatattctca atctcaccat tcgcggtcag
//
