LOCUS       sequence-103769-         782 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  PhoRadAdN38 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-467
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             60..82
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(735..752)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agaaataatt
       61 ttgtttaact ttaagaagga gatatacata tgaatggtta cgcagttcca ttagataatg
      121 tctttgtgta cacgttggac atcgctagtg gtgaaattaa gaaaacgagg gcttcataca
      181 tctatcgcga gaaggttgag aagctcattg agataaaact atccagtgga tactccctta
      241 aggttactcc ctctcaccca gttctcctct ttagagatgg cctgcaatgg gtccccgctg
      301 ctgaagttaa acctggagat gttgttgttg gtgttaggga agaggtactg agaagaagaa
      361 taatatccaa aggagaactt gaattccatg aggtttcctc ggtaaggata atagattaca
      421 ataactgggt ctacgacctt gtaattccgg aaacccacaa cttcatagct cccaatggac
      481 ttgttctcca taatactcag ctaggtaccc agtacaaact tatcctgaac ggtaaaaccc
      541 tgaaaggtga aaccaccacc gaagctgttg acgctgctac cgcggaaaaa gttttcaaac
      601 agtacgctaa cgacaacggt gttgacggtg aatggaccta cgacgacgct accaaaacct
      661 acaccgtaac ggaattccat catcatcatc atcattaagc ttggctgttt tggcggatga
      721 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
      781 aa
//
