LOCUS       sequence-103804-         439 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes cytoplasmic dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-R
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..43)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(229..247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggatctagga taacttcgta taggatactt tatacgaagt tattgatcaa ttcacagcca
       61 ccatgggagc gccagctgga ggtgcaggtg cagcaatggt gagcaagggc gaggaggtca
      121 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
      301 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
      421 cccaggactc ctccctgca
//
