LOCUS       sequence-103861-         885 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  PhoRadAN38 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-459
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(212..502)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(509..526)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    582..606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(606..625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(607..625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        671..748
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(768..787)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttaat cgaatggtaa ttccccattc atcgaggcgt
      121 atttactata catctcctca attgattcca tgtgtggtac agtatcgttt tcataataaa
      181 cttcggtatc cctagcaaag cacttcccgg atccaccaat ctgttctctg tgagtctcaa
      241 taatatcgtt atcctccatg tccaaatctt caggggtctg atcagcttga attctaatac
      301 cgtcgtacaa gaatcttaag gagtccattt ccttaccctg tcttttagcg aacgcttcca
      361 tcagccttct taaaggagtg gtctttttga tcttaaagaa gatttctgaa gatccatcgg
      421 acacctttaa attgatgtga gtctcaggct tgacttctgg cttgacctct ggcttagctt
      481 cttgattgac ttctgagtca ctagtgctgt gatgatgatg atgatggctg ctgcccatgg
      541 tatatctcct tattaaagtt aaacaaaatt atttctacag gggaattgtt atccgctcac
      601 aattccccta tagtgagtcg tattaatttc ctaatgcagg agtcgcataa gggagagcgt
      661 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
      721 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
      781 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      841 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaa
//
