LOCUS       sequence-103868-         843 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  NpuDnaB(delta283)dC39 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-259
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..366
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             695..739
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(777..795)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      791..838
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtaaataat tttgtttaac tttaataagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcactagtga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca
      121 gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agaaatcttc
      181 tttaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag
      241 ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag
      301 acccctgaag atttggacat ggaggataac gatattattg agactcacag agaacagatt
      361 ggtggatccg gttgtttagc aggcgatagt ctggtgacat tagtagatag cggactgcaa
      421 gtgccaatta aggaattagt aggtaaatct ggttttgcag tttgggcatt gaacgaagct
      481 acaatgcagc tagaaaaggc aattgttagc aatgcttttt cgactggtat aaagcctctg
      541 tttactttga caactcgatt ggggcggaaa atccgtgcca caggtaatca caagttttta
      601 acaattaatg gctggaaaag acttgatgaa ttaactccta aagaacatct cagtttacca
      661 agaaattaag cttgcggccg cactcgagtc tggtaaagaa accgctgctg cgaaatttga
      721 acgccagcac atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc
      781 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      841 gaa
//
