LOCUS       sequence-104011-         965 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  IncP-based replicating vector expressing dTomato under tac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14."
COMMENT     Sequence Label: pAWP89
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(208..909)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(291..314)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(489..508)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     758..776
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(join(938..944,945..960,961..965))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
       61 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
      121 cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg agtggaagga
      181 tcagatcacg catcttcccg acaactactt gtacagctcg tccatgccgt acaggaacag
      241 gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
      301 ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc agttgcacgg gcttcttggc
      361 catgtagatg gtcttgaact ccaccaggta gtggccgccg tccttcagct tcagggcctg
      421 gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca
      481 gcccatggtc ttcttctgca ttacggggcc gtcggggggg aagttggtgc cgcgcatctt
      541 caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc
      601 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt
      661 gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg gagccgtaca tgaactgggg
      721 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt
      781 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
      841 catggagccc tccatgcgca ccttgaagcg catgaactct ttgatgacct cctcgccctt
      901 gctcaccata gctgtttcct gtgtgaatac ctccacacat tatacgagcc gatgattaat
      961 tgtca
//
