LOCUS       sequence-104115-         893 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  To construct C-terminal GFP fusions, single crossover integration 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pAE03
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     563..591
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     misc_feature    630..686
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(699..715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(699..715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(712..734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    723..739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..771,772..777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    792..813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aagggcagat tgtgtcgaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
       61 tggagtattt tgttgataat ggtctgctag ttgaacggat ccatcttcaa tgttgtggcg
      121 aattttgaag ttagctttga ttccattctt ttgtttgtct gccgtgatgt atacattgtg
      181 tgagttatag ttgtactcga gtttgtgtcc gagaatgttt ccatcttctt taaaatcaat
      241 accttttaac tcgatacgat taacaagggt atcaccttca aacttgactt cagcacgcgt
      301 cttgtagttc ccgtcatctt tgaaagatat agtgcgttcc tgtacataac cttcgggcat
      361 ggcactcttg aaaaagtcat gccgtttcat atgatccgga taacgggaaa agcattgaac
      421 accataggtc aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca
      481 aataaattta agggtaagct ttccgtatgt agcatcacct tcaccctctc cactgacaga
      541 aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      601 aagttcttct cctttgctag cgcggccgcg aattcgagct cggtacccgg ggatcctcta
      661 gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
      721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggg
//
