LOCUS       sequence-104454-        1077 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, DsRed-NLS marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSARN
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(243..276)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    324..458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(348..367)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     402..421
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(599..619)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(688..711)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtactc
      181 gagctccacc gcggtggcgg ccgctctagg cgcgccgtac gggctgcagg tcgagggacc
      241 taataacttc gtatagcata cattatacga acggtaatta agggttccgg atccccatca
      301 agcttatcga taccgtcaaa cgggatccag acatgataag atacattgat gagtttggac
      361 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      421 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      481 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      541 aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcttac gggtcctcca
      601 ccttccgctt tttcttgggt cgagatctca ggaacaggtg gtggcggccc tcggtgcgct
      661 cgtactgctc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggcgtcca
      721 cgtagtagta gccgggcagc tgcacgggct tcttggccat gtagatggac ttgaactcca
      781 ccaggtagtg gccgccgtcc ttcagcttca gggccttgtg ggtctcgccc ttcagcacgc
      841 cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatca
      901 cggggccgtc ggaggggaag ttcacgccga tgaacttcac cttgtagatg aagcagccgt
      961 cctgcaggga ggagtcctgg gtcacggtcg ccacgccgcc gtcctcgaag ttcatcacgc
     1021 gctcccactt gaagccctcg gggaaggaca gcttcttgta gtcggggatg tcggcgg
//
