LOCUS       sequence-104456-        1037 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  phageC31 integrase-expressing helper plasmid for Anopheles 
            transgenesis, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR R4-vas2-integrase-R3
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(102..225)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagta caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt
      121 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      181 ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgtctcg agaaaatgtg
      241 gccattaaca gcagtttttt ttaaaacgat gtatcaacca tctaacatgc atattttaca
      301 attttcagga aaataatcaa gctctaatat tgtactaaag aaacatatag atatagtaat
      361 aatactatag atatagaaat ttcaaatatt ctcctttttg gaatagttag acggtcatgg
      421 tggcctatac tggcttttgg aatttactga gtttacgccg ccagttacaa gggatcggtc
      481 catttgaggt aagaaccctc gatgggcatg ttgtcaagtc tagcagtatg acgtttgtac
      541 catggtaccg ctccgtaaat tgaatatgtt attagtattt tcttagccaa acgagacata
      601 aacgataagc cactagataa aggatacatc aagatggtgc tctaagcggc ccgcgggctg
      661 ataataaagt ttgaagaccc ctgaacgata tgttgacaat gaacgggctt tgtgcagtta
      721 cgctcaattc gtcgttatct tttttgaaac ctgggttagt ctaagtactt tgtacaacta
      781 ccttcacttg tgtattttat cctggaatgg acctaaaggt gacgtattgt tagaatactt
      841 cagttctaga gtatacaagt gcgaaaaatc acctttcagt acattacatt gaataatgaa
      901 agacaatcgg tgcaagcttt cacgaattat agaataaaag tgcaagttct tatgttttca
      961 aagttttcat caacgtttta ttaacgaaca attctttgct gtgtgtcatg cttcttctgt
     1021 gtgttttaca tcgttac
//
